Product Name	PSD93/DLG2
Chinese Name	突触后密度蛋白93Recombinant rabbit monoclonal anti
Alias	Channel associated protein of synapse 110; Channel associated protein of synapses 110kD; Channel-associated protein of synapse-110; Chapsyn 110; Chapsyn-110; Chapsyn110; discs large homolog 2; Discs, large homolog 2 (Drosophila); Disks large homolog 2; DKFZp781D1854; DKFZp781E0954; Dlg 2; dlg2; DLG2_HUMAN; Dlgh 2; Dlgh2; FLJ37266; Gm1197; MGC131811; Postsynaptic density protein PSD 93; Postsynaptic density protein PSD-93; Postsynaptic density protein PSD93; PSD 93; PSD93.
Research Area	Cell biology  immunology  Neurobiology  Signal transduction
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	6D1
React Species	Human,  (predicted: Mouse, Rat, )
Applications	WB=1:500-2000 IP=1:10-50 Flow-Cyt=1:50  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	98kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PSD93/DLG2
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	PSD 93 is believed to participate in the clustering of certain proteins, including N-methyl-D-aspartate (NMDA) receptors and shaker-type potassium channels at the synaptic membrane. There are two principal modes of interaction between PSD 93 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with PSD 93 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD 93 in vitro through a pseudo-homotypic PDZ-PDZ interaction. Function: Required for perception of chronic pain through NMDA receptor signaling. Regulates surface expression of NMDA receptors in dorsal horn neurons of the spinal cord. Interacts with the cytoplasmic tail of NMDA receptor subunits as well as inward rectifying potassium channels. Involved in regulation of synaptic stability at cholinergic synapses. Part of the postsynaptic protein scaffold of excitatory synapses. Subunit: Interacts through its PDZ domains with NETO1. Interacts with NOS1/nNOS through second PDZ domain. Interacts with KCNJ2/Kir2.1 (via C-terminus) through one of its PDZ domains. Interacts with FRMPD4 (via C-terminus). Interacts with LRFN1, LRFN2 and LRFN4. Interacts with FASLG. Subcellular Location: Membrane; Lipid-anchor. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Note=Concentrated in soma and postsynaptic density of a subset of neurons. Post-translational modifications: Palmitoylation of isoform 1 is not required for targeting to postsynaptic density. Similarity: Belongs to the MAGUK family. Contains 1 guanylate kinase-like domain. Contains 3 PDZ (DHR) domains. Contains 1 SH3 domain. SWISS: Q15700 Gene ID: 1740 Database links: Entrez Gene: 1740 Human Entrez Gene: 23859 Mouse Entrez Gene: 64053 Rat Omim: 603583 Human SwissProt: Q15700 Human SwissProt: Q91XM9 Mouse SwissProt: Q63622 Rat Unigene: 367656 Human Unigene: 147213 Mouse Unigene: 257035 Mouse Unigene: 202966 Rat
Product Picture	Sample: Lane 1: Hela cell lysate Lane 2: SH-SY5Y cell lysate Primary: Anti-PSD93/DLG2 (SLM-54145R) at 1:500 dilution Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution Predicted band size: 98 kD Observed band size: 100 kD Blank control:SH-SY5Y. Primary Antibody (green line): Rabbit Anti-PSD93 antibody (SLM-54145R) Dilution: 1:50; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1:1000. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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