Product Name	MMP9
Chinese Name	基质金属蛋白酶-9Recombinant rabbit monoclonal anti
Alias	Matrix metalloproteinase-9 precursor; MMP-9; MMP9; MMP 9; 92 kDa type IV; Collagenase; 92 kDa gelatinase; Gelatinase B; GELB; MMP9_HUMAN; 82 kDa matrix metalloproteinase-9; 92 kDa type IV collagenase; CLG 4B; CLG-4B; CLG4B; Collagenase Type 4 beta; Collagenase Type-4 beta; Collagenase type IV 92 KD; Collagenase type IV 92 KD; EC 3.4.24.35; Gelatinase 92 KD; Gelatinase 92 KD; Gelatinase beta; Gelatinase-beta; GelatinaseB; GELB; Macrophage gelatinase; MANDP2; Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); Matrix Metalloproteinase 9; Type V collagenase.
literatures	Specific References  (1)     |     SLM-54040R has been referenced in 1 publications. [IF=5.988] Wenjun He. et al. Molecular Mechanism of Naringenin Against High-Glucose-Induced Vascular Smooth Muscle Cells Proliferation and Migration Based on Network Pharmacology and Transcriptomic Analyses.. FRONT PHARMACOL. 2022 Jun;13:862709-862709  IHC, WB ;  Mouse.   PubMed:35754483
Research Area	Tumour  Cell biology  Neurobiology  Signal transduction  Apoptosis
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	7D6
React Species	Human, Rat,  (predicted: Mouse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:50-200 IHC-F=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	100kDa
Cellular localization	Extracellular matrix Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human MMP9
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008] Function: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Subunit: Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1. Subcellular Location: Secreted; extracellular space; extracellular matrix. Tissue Specificity: Produced by normal alveolar macrophages and granulocytes. Post-translational modifications: Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9. DISEASE: Defects in MMP9 are the cause of metaphyseal anadysplasia type 2 (MANDP2) [MIM:613073]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia. Similarity: Belongs to the peptidase M10A family. Contains 3 fibronectin type-II domains. Contains 4 hemopexin-like domains. SWISS: P14780 Gene ID: 4318 Database links: Entrez Gene: 403885 Dog Entrez Gene: 4318 Human Entrez Gene: 17395 Mouse Entrez Gene: 81687 Rat Omim: 120361 Human SwissProt: O18733 Dog SwissProt: P14780 Human SwissProt: P41245 Mouse Synthesis and Degradation（Synthesis and Degradation） MMP9亦称IV型胶原酶或明胶酶B，其主要功能为降解IV型胶原。因而它在Tumour细胞突破基底膜屏障和浸润转移中起重要作用。 目前主要用于各种恶性Tumour(如乳腺癌、胃肠道癌、卵巢癌、膀胱癌等)中的基底膜检测与转移浸润的研究。Extracellular matrix在维持正常组织结构与功能以及细胞生长和分化过程中起重要作用。Extracellular matrix动态平衡的失调与Tumour细胞侵袭、转移和复发密切相关，基质金属蛋白酶(MMP-9)是Extracellular matrix的降解酶，可降解Ⅳ、Ⅴ、Ⅸ、Ⅺ型胶原，在Tumour的浸润、转移过程中起重要作用，近年为Tumour研究的热点。 Kiss-1与MMP-9、MMP-2 是转移浸润研究的热门课题。
Product Picture	Sample: Lane 1: Normal human U-2 OS cell lysates Lane 2: U-2 OS treated with 200nM TPA for 48 hours Lane 3: Normal human MDA-MB-231 cell lysates Lane 4: MDA-MB-231 serum starved overnight, then treated with 200nM TPA for 24 hours Primary: Anti-MMP9 (SLM-54040R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100 kDa Observed band size: 82 kDa Sample: Lane 1: Spleen (Rat) Lysate at 40 ug Lane 2: Lung (Rat) Lysate at 40 ug Primary: Anti-MMP9 (SLM-54040R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 92/82-85 kD Observed band size: 90 kD Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MMP9) Monoclonal Antibody, Unconjugated (SLM-54040R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MMP9) Monoclonal Antibody, Unconjugated (SLM-54040R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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