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Product Name NQO1 Chinese Name 醌氧化还原酶Recombinant rabbit monoclonal anti Alias Azoreductase; Cytochrome b 5 reductase; DHQU; DIA 4; DIA4; Diaphorase (NADH/NADPH) (cytochrome b 5 reductase); Diaphorase (NADH/NADPH) (cytochrome b-5 reductase); Diaphorase (NADH/NADPH) (cytochrome b-5 reductase); Diaphorase (NADH/NADPH); Diaphorase 4; Dioxin inducible 1; DT diaphorase; DT-diaphorase; DTD; Menadione reductase; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase quinone 1; NAD(P)H menadione oxidoreductase 1 dioxin inducible; NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; NMOR 1; NMOR I; NMOR1; NMORI; NQO 1; NQO1_HUMAN; Phylloquinone reductase; Phylloquinone reductase; QR 1; QR1; Quinone reductase 1; Quinone reductase 1. literatures Specific References (1) | SLM-52830R has been referenced in 1 publications.[IF=1.789] Zeba Farooqui . et al. Nrf2 inhibition induces oxidative stress, renal inflammation and hypertension in mice. Clin Exp Hypertens. 2021;43(2):175-180 WB ; Mouse.Research Area Tumour Signal transduction Synthesis and Degradation The new supersedes the old Immunogen Species Rabbit Clonality Monoclonal Clone NO. 8T5 React Species Human, Mouse, (predicted: Rat, ) Applications WB=1:1000-3000 IP=1:20-100 Flow-Cyt=1:50 ICC=1:100-500 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 31kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human NQO1 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].
Function:
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Subunit:
Homodimer.
Subcellular Location:
Cytoplasm.
Similarity:
Belongs to the NAD(P)H dehydrogenase (quinone) family.
SWISS:
P15559
Gene ID:
1728
Database links:
Entrez Gene: 1728 Human
Entrez Gene: 18104 Mouse
Omim: 125860 Human
SwissProt: P15559 Human
SwissProt: Q64669 Mouse
Unigene: 406515 Human
Unigene: 252 Mouse
Unigene: 11234 Rat
Product Picture Sample:
Lane 1: mouse kidney tissue lysate
Lane 2: SH-SY5Y cell lysate
Primary: Anti-NQO1 (SLM-52830R) at 1:500 dilution
Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-NQO1 antibody (SLM-52830R)
Dilution: 1:100;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1:1000.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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