Product Name	Tissue factor
Chinese Name	组织因子（CD142）兔单克隆抗体
Alias	CD 142; CD-142; CD142; CD142 antigen; Coagulation factor III (thromboplastin tissue factor); Coagulation factor III; F3; TF; TFA; Thromboplastin; Tissue factor; TF_HUMAN.
literatures	Specific References  (1)     |     SLM-52647R has been referenced in 1 publications. [IF=5.118] Masahiro Terasawa. et al. Anti-Inflammatory Activity of Orally Administered Monostroma nitidum Rhamnan Sulfate against Lipopolysaccharide-Induced Damage to Mouse Organs and Vascular Endothelium. Mar Drugs. 2022 Feb;20(2):121  WB ;  Mouse.   PubMed:10.3390/md20020121
Research Area	Cardiovascular  Cell biology  immunology  Growth factors and hormones  Cell Surface Molecule
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	6A4
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 IHC-P=1:100-500 ICC=1:50 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	35kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Tissue factor: 1-100/295
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010] Function: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. Subunit: Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII. Subcellular Location: Isoform 1: Membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Tissue Specificity: Lung, placenta and pancreas. Similarity: Belongs to the tissue factor family. SWISS: P13726 Gene ID: 2152 Database links: Entrez Gene: 2152 Human Entrez Gene: 14066 Mouse Entrez Gene: 25584 Rat Omim: 134390 Human SwissProt: P13726 Human SwissProt: P20352 Mouse SwissProt: P42533 Rat Unigene: 62192 Human Unigene: 273188 Mouse Unigene: 9980 Rat
Product Picture	Sample: Lane 1: Mouse Brain Lysates Lane 2: Rat Kidney Lysates Lane 3: Human A431 cell Lysates Lane 4: Human MDA-MB-231 cell Lysates Lane 5: Human U-87 MG cell Lysates Lane 6: Human PANC-1 cell Lysates Primary: Anti-Tissue factor (SLM-52647R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35kDa Observed band size: 45kDa Sample: Lane 1: mouse brain tissue lysate Lane 2: U937 cell lysate Lane 3: SH-SY5Y cell lysate Primary: Anti-Tissue factor (SLM-52647R) at 1:500 dilution Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution Predicted band size: 35 kD Observed band size: 50 kD Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tissue factor) Monoclonal Antibody, Unconjugated (SLM-52647R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tissue factor) Monoclonal Antibody, Unconjugated (SLM-52647R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tissue factor) Monoclonal Antibody, Unconjugated (SLM-52647R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. SHG-44 Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (Tissue Factor) monoclonal Antibody, Unconjugated (SLM-52647R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (Tissue Factor) monoclonal Antibody, Unconjugated (SLM-52647R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. PANC-1 Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (Tissue Factor) monoclonal Antibody, Unconjugated (SLM-52647R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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