Product Name	Cyclooxygenase 2
Chinese Name	环氧合酶2Recombinant rabbit monoclonal anti
Alias	PTGS2; COX 2; COX2; COX-2; Cyclooxygenase; Cyclooxygenase2; Cyclooxygenase-2; hCox 2; PGG/HS; PGH synthase 2; PGHS 2; PGHS2; PHS 2; PHS II; PHS2 ; Prostaglandin endoperoxide synthase 2; Prostaglandin G/H synthase 2 precursor; Prostaglandin G/H synthase and cyclooxygenase; Prostaglandin G/H synthase 2; Prostaglandin H2 synthase 2; TIS10II; PGH2_HUMAN.  前列腺素内过氧化物合成酶2
literatures	Specific References  (2)     |     SLM-52502R has been referenced in 2 publications. [IF=4.636] Chen Jiahao. et al. Roles of glutathione peroxidase 4 on the mercury-triggered ferroptosis in renal cells: Implications for the antagonism between selenium and mercury. METALLOMICS. 2023 Mar;:  WB ;  Human.   PubMed:36869799 [IF=4.225] Ye Xietao. et al. How Aconiti Radix Cocta can Treat Gouty Arthritis Based on Systematic Pharmacology and UPLC-QTOF-MS/MS. Front Pharmacol. 2021 Apr;12:971  WB ;  Human.   PubMed:33995019
Research Area	Tumour  Cardiovascular  immunology  Signal transduction  Synthesis and Degradation  The new supersedes the old
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	14D5
React Species	Human,  (predicted: Mouse, )
Applications	WB=1:500-2000 IHC-P=1:400-800 ICC=1:50 IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	65kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human COX2 protein
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009] Function: Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. Subunit: Homodimer. Subcellular Location: Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Post-translational modifications: S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. Similarity: Belongs to the prostaglandin G/H synthase family. Contains 1 EGF-like domain. SWISS: P35354 Gene ID: 5743 Database links: Entrez Gene: 5743 Human Entrez Gene: 19225 Mouse Entrez Gene: 29527 Rat Omim: 600262 Human SwissProt: P35354 Human SwissProt: Q05769 Mouse SwissProt: P35355 Rat Unigene: 196384 Human Unigene: 292547 Mouse Unigene: 44369 Rat
Product Picture	Sample: Lane 1: A549 cell lysates Primary: Anti-COX2/Cyclooxygenase 2 (SLM-52502R) at 1:500 dilution Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution Predicted band size: 65 kD Observed band size: 65 kD Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cyclooxygenase 2) Monoclonal Antibody, Unconjugated (SLM-52502R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cyclooxygenase 2) Monoclonal Antibody, Unconjugated (SLM-52502R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (COX2/Cyclooxygenase 2) monoclonal Antibody, Unconjugated (SLM-52502R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (COX2/Cyclooxygenase 2) monoclonal Antibody, Unconjugated (SLM-52502R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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