Product Name	Bax
Chinese Name	BaxRecombinant rabbit monoclonal anti
Alias	apoptosis regulator BAX; Apoptosis regulator BAX cytoplasmic isoform beta; Apoptosis regulator BAX membrane isoform alpha; Bax isoform psi; BAX protein cytoplasmic isoform delta; Bax protein cytoplasmic isoform delta. antibody Bax protein cytoplasmic isoform gamma; Bax zeta; Bax-protein; Bcl-2-like protein 4; BCL2 associated X protein; BCL2L4; BAX_HUMAN; Bcl2-L-4.
literatures	Specific References  (10)     |     SLM-52316R has been referenced in 10 publications. [IF=5.895] Yu-Sheng Shi. et al. Pteris laeta Wall. and Its New Phytochemical, Pterosinsade A, Promote Hippocampal Neurogenesis via Activating the Wnt Signaling Pathway. J AGR FOOD CHEM. 2023;XXXX(XXX):XXX-XXX  WB ;  Mouse.   PubMed:36892329 [IF=5.722] Wang, Sanchun. et al. Pseudoginsengenin DQ exerts antitumour activity against hypopharyngeal cancer cells by targeting the HIF-1α-GLUT1 pathway. Cancer Cell Int. 2021 Dec;21(1):1-12  WB ;  Human.   PubMed:34281558 [IF=5.572] Miao Song. et al. Mitophagy alleviates AIF-mediated spleen apoptosis induced by AlCl3 through Parkin stabilization in mice. FOOD CHEM TOXICOL. 2023 Jun;176:113762  WB ;  Mouse.   PubMed:37028746 [IF=4.472] Fangji Zhou. et al. Mono-(2-ethylhexyl) Phthalate (MEHP)-Induced Telomere Structure and Function Disorder Mediates Cell Cycle Dysregulation and Apoptosis via c-Myc and Its Upstream Transcription Factors in a Mouse Spermatogonia-Derived (GC-1) Cell Line. TOXICS. 2023 May;11(5):448  WB ;  Mouse.   PubMed:37235262 [IF=4.36] Zhuohong Li. et al. Anti-inflammatory and anti-apoptotic effects of Shaoyao decoction on X-ray radiation-induced enteritis of C57BL/6 mice. J Ethnopharmacol. 2022 Mar;:115158  WB ;  Mouse.   PubMed:35245630 [IF=4.36] Xiaojing Zhang. et al. A new discovery: Total Bupleurum saponin extracts can inhibit the proliferation and induce apoptosis of colon cancer cells by regulating the PI3K/Akt/mTOR pathway. J Ethnopharmacol. 2022 Jan;283:114742  WB ;  Human.   PubMed:34655668 [IF=4.171] Chenying Gao. et al. Inhibition of the Ras/ERK1/2 pathway contributes to the protective effect of ginsenoside Re against intimal hyperplasia. 2021 May 20  IHC ;  Rat.   PubMed:34116563 [IF=3.647] Shuang Huet al. MicroRNA-708 prevents ethanol-induced hepatic lipid accumulation and inflammatory reaction via direct targeting ZEB1. Life Sci . 2020 Oct 1;258:118147.  WB ;  Human.   PubMed:32721464 [IF=2.447] Yanji Zhu. et al. P2X7R antagonist protects against renal injury in mice with adriamycin nephropathy. Exp Ther Med. 2022 Feb;23(2):1-8  IHC ;  Mouse.   PubMed:35069842 [IF=1.73] Chao Wang. et al. Inhibition of monoacylglycerol lipase restrains proliferation, migration, invasion, tumor growth and induces apoptosis in cervical cancer. 2021 Dec 07  WB ;  Human.   PubMed:34877750
Research Area	Cell biology  Signal transduction  Apoptosis
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	3C3
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:50-200 ICC=1:100 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	21kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Bax
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]. Function: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. Subunit: Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L). Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2. Subcellular Location: Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Note=Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane. Isoform Beta: Cytoplasm. Isoform Gamma: Cytoplasm. Isoform Delta: Cytoplasm (Potential). Tissue Specificity: Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. Similarity: Belongs to the Bcl-2 family. SWISS: Q07812 Gene ID: 581 Database links: Entrez Gene: 581 Human Entrez Gene: 12028 Mouse Entrez Gene: 24887 Rat Omim: 600040 Human SwissProt: Q07812 Human SwissProt: Q07813 Mouse SwissProt: Q63690 Rat Unigene: 624291 Human Unigene: 19904 Mouse Unigene: 10668 Rat
Product Picture	Sample: Testis (Mouse) Lysate at 40 ug Testis (Rat) Lysate at 40 ug Spleen (Mouse) Lysate at 40 ug Spleen (Rat) Lysate at 40 ug Lung (Mouse) Lysate at 40 ug Lung (Rat) Lysate at 40 ug MCF-7(Human) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug Raji(Human) Cell Lysate at 30 ug Primary: Anti-Bax (SLM-52316R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18'21 kD Observed band size: 21 kD SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bax) monoclonal Antibody, Unconjugated (SLM-52316R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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