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Product Name Alkaline phosphatase, tissue-nonspecific isozyme Chinese Name 碱性磷酸酶Recombinant rabbit monoclonal anti Alias ALPL; AKP2; Alkaline phosphatase liver/bone/kidney; Alkaline phosphatase liver/bone/kidney isozyme; Alkaline phosphatase tissue nonspecific isozyme; AP TNAP; AP-TNAP; APTNAP; BALP; BAP; FLJ40094; FLJ93059; Glycerophosphatase; HOPS; Liver/bone/kidney isozyme; Liver/bone/kidney type alkaline phosphatase; MGC161443; MGC167935; PHOA; PPBT_HUMAN; Tissue non specific alkaline phosphatase; Tissue nonspecific ALP; TNAP; TNSALP. literatures Specific References (4) | SLM-52252R has been referenced in 4 publications.[IF=11.322] Jinxiu Yu. et al. Promoting osseointegration of titanium by pH-responsive releasing of H2S and optimizing polarization time for macrophages. COMPOS PART B-ENG. 2023 Mar;253:110554 IHC ; Rat.[IF=7.032] Mengmeng Liang. et al. Osteoclast-derived small extracellular vesicles induce osteogenic differentiation via inhibiting ARHGAP1. Mol Ther-Nucl Acids. 2021 Mar;23:1191 WB ; Mouse.[IF=5.875] Jie Kong. et al. Safflower oil body nanoparticles deliver hFGF10 to hair follicles and reduce microinflammation to accelerate hair regeneration in androgenetic alopecia. Int J Pharmaceut. 2022 Mar;616:121537 IF ; Mouse.[IF=4.147] Yang Zhang. et al. Transcriptome sequencing profiling identifies miRNA-331-3p as an osteoblast-specific miRNA in infected bone nonunion. Bone. 2021 Feb;143:115619 WB ; Mouse.Research Area Cell biology immunology Kinases and Phosphatases Immunogen Species Rabbit Clonality Monoclonal Clone NO. 1G9 React Species Human, Mouse, Rat, Applications WB=1:500-2000 IP=1:20-50 IHC-P=1:100-500 IHC-F=1:50-100 ICC=1:50-100 IF=1:50-100 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 56kDa Cellular localization The nucleus cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Alkaline Phosphatase: 18-50 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
Function:
This isozyme may play a role in skeletal mineralization.
Subunit:
Homodimer.
Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor.
Post-translational modifications:
Glycosylated.
DISEASE:
Defects in ALPL are a cause of hypophosphatasia (HOPS) [MIM:146300]. HOPS is an inherited metabolic bone disease characterized by defective skeletal mineralization. Four hypophosphatasia forms are distinguished, depending on the age of onset: perinatal, infantile, childhood and adult type. The perinatal form is the most severe and is almost always fatal. Patients with only premature loss of deciduous teeth, but with no bone disease are regarded as having odontohypophosphatasia (odonto).
Defects in ALPL are a cause of hypophosphatasia childhood type (HOPSC) [MIM:241510].
Defects in ALPL are a cause of hypophosphatasia infantile type (HOPSI) [MIM:241500].
Similarity:
Belongs to the alkaline phosphatase family.
SWISS:
P05186
Gene ID:
249
Database links:Entrez Gene: 249 Human
Entrez Gene: 11647 Mouse
Omim: 171760 Human
SwissProt: P05186 Human
SwissProt: P09242 Mouse
Unigene: 75431 Human
Unigene: 288186 Mouse
Unigene: 82764 Rat
Product Picture Sample:
Lane 1: Placenta (Mouse) Lysate at 40 ug
Lane 2: Liver (Mouse) Lysate at 40 ug
Lane 3: Cerebrum (Mouse) Lysate at 40 ug
Lane 4: Lung (Mouse) Lysate at 40 ug
Lane 5: Kidney (Mouse) Lysate at 40 ug
Lane 6: Liver (Rat) Lysate at 40 ug
Lane 7: Lung (Rat) Lysate at 40 ug
Lane 8: Kidney (Rat) Lysate at 40 ug
Lane 9: Hela (Human) Cell Lysate at 30 ug
Lane 10: MCF-7 (Human) Cell Lysate at 30 ug
Lane 11: U2os (Human) Cell Lysate at 30 ug
Lane 12: MG63 (Human) Cell Lysate at 30 ug
Lane 13: JEG-3 (Human) Cell Lysate at 30 ug
Primary: Anti-Alkaline Phosphatase (SLM-52252R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 72 kD
Tissue: Human liver
Section type: Formalin-fixed & Paraffin
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal
for SLM-60665R
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