Product Name	Oct4
Chinese Name	胚胎Stem cells关键蛋白Recombinant rabbit monoclonal anti
Alias	MGC22487; Oct 3; Oct 4; Oct-3; Oct-4; OCT3; Oct4; Octamer binding protein 3; Octamer-binding protein 3; Octamer binding protein 4; Octamer binding transcription factor 3; Octamer-binding transcription factor 3; Octamer-4; POU domain transcription factor OCT4; OTF3; OTF4; OTF 3; OTF 4; OTF-3; POU class 5 homeobox 1; POU domain, class 5, transcription factor 1; POU domain class 5 transcription factor 1; PO5F1_HUMAN; POU type homeodomain containing DNA binding protein ; POU5F1.
literatures	Specific References  (1)     |     SLM-52002R has been referenced in 1 publications. [IF=2.13] Jijiong Zhang. et al. CircRNA hsa_circ_0075048 promotes the malignant progression of non-small cell lung cancer by up-regulating HMGB2 expression via targeting miR-1225-5p.. HISTOL HISTOPATHOL. 2022 Nov;:18551-18551  WB ;  Human.   PubMed:36416408
Research Area	Cell biology  Developmental biology  Signal transduction  Stem cells  Cyclin  transcriptional regulatory factor  Cell type markers  Epigenetics
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	2D5
React Species	(predicted: Human, Mouse, Rat, )
Applications	WB=1:500-2000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:50-200 ICC=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	39kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human Oct 4 protein, around 1-100aa
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state. Function: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Subunit: Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2. Subcellular Location: Nucleus.Note=Expressed in a diffuse and slightly punctuate pattern. Tissue Specificity: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. Post-translational modifications: Belongs to the POU transcription factor family. Class-5 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain. Similarity: Belongs to the POU transcription factor family. Class-5 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain. SWISS: Q01860 Gene ID: 5460 Database links: Entrez Gene: 282316 Cow Entrez Gene: 5460 Human Entrez Gene: 18999 Mouse Entrez Gene: 100127461 Pig Entrez Gene: 294562 Rat Omim: 164177 Human SwissProt: O97552 Cow SwissProt: Q01860 Human SwissProt: P20263 Mouse SwissProt: Q9TSV5 Pig Unigene: 249184 Human Unigene: 632482 Human Unigene: 646545 Human Unigene: 17031 Mouse Unigene: 161748 Rat
Product Picture	Western blot analysis of Oct4 on F9 cell lysates with Rabbit anti-Oct4 antibody (SLM-52002R) at 1/1,000 dilution. Lysates/proteins at 10 碌g/Lane. Predicted band size: 39 kDa Observed band size: 45 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (SLM-52002R) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature. Western blot analysis of Oct4 on different lysates with Rabbit anti-Oct4 antibody (SLM-52002R) at 1/500 dilution. Lane 1: HES cell lysate Lane 2: NCCIT cell lysate Lysates/proteins at 10 碌g/Lane. Predicted band size: 39 kDa Observed band size: 45 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (SLM-52002R) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature. Immunohistochemical analysis of paraffin-embedded human seminoma tissue tissue with Rabbit anti-Oct4 antibody (ET1612-20) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (SLM-52002R) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Oct4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (SLM-52002R, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. ICC staining of Oct4 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (SLM-52002R, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). ICC staining of Oct4 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (SLM-52002R, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

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