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Product Name HIF1AN Chinese Name 缺氧诱导因子1α抑制蛋白/HIF1AN单克隆抗体 Alias Hypoxia inducible factor asparagine hydroxylase; Factor inhibiting HIF1; FIH 1; FIH-1; FIH1; Hypoxia inducible factor 1 alpha inhibitor; Hypoxia inducible factor 1 alpha subunit inhibitor; Peptide aspartate beta dioxygenase; DKFZp762F1811; FLJ20615; FLJ22027; HIF1N_HUMAN. Research Area Tumour Cardiovascular Cell biology Chromatin and nuclear signals Neurobiology Signal transduction transcriptional regulatory factor Immunogen Species Mouse Clonality Monoclonal Clone NO. L8U9 React Species Human, Applications WB=1:500-2000 ICC=1:20-50 IF=1:20-50
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 38kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human HIF1AN: 1-349/349 Lsotype IgG1,k Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail HIF1AN (Hypoxia inducible factor 1 alpha inhibitor) is a corepressor that interacts with hypoxia inducible factor 1 (HIF1) alpha and the von Hippel Lindau tumor suppressor protein to mediate repression of HIF1 transcriptional activity.
Function:
Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminaltransactivation domain (CAD). Functions as an oxygen sensor and,under normoxic conditions, the hydroxylation prevents interactionof HIF-1 with transcriptional coactivators includingCbp/p300-interacting transactivator. Involved in transcriptionalrepression through interaction with HIF1A, VHL and histonedeacetylases. Hydroxylates specific Asn residues within ankyrinrepeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A andseveral other ARD-containing proteins. Also hydroxylates Asp andHis residues within ARDs of ANK1 and TNKS2, respectively.Negatively regulates NOTCH1 activity, accelerating myogenicdifferentiation. Positively regulates ASB4 activity, promotingvascular differentiation.
Subunit:
Homodimer; homodimerization is essential for catalyticactivity. Interacts with VHL and HIF1A. Part of a complex with VHL,HIF1A and HDAC1 or HDAC2 or HDAC3. Interacts with NFKB1 and NFKBIA.Interacts with NOTCH1, NOTCH2 and NOTCH3 but not with NOTCH4.Interacts with APBA3; binding inhibits HIF1AN binding to HIF1A.Interacts with TNKS2. Interacts with PPP1R12A. Interacts with ASB4(By similarity). Interacts with UBE3A.
Subcellular Location:
Nucleus. Cytoplasm. Cytoplasm, perinuclearregion. Note=Mainly cytoplasmic localization, but interaction withNOTCH1 results in nuclear localization and interaction with ABPA3results in perinuclear localization in macrophages.
Similarity:
Contains 1 JmjC domain.
SWISS:
Q9NWT6
Gene ID:
55662
Database links:Entrez Gene: 55662 Human
SwissProt: Q9NWT6 Human
Product Picture Sample:
Lane 1: 293 cell lysates
Lane 2: Jurkat cell lysates
Lane 3: ZR-75-1 cell lysates
Primary: Anti-HIF1AN (SLM-51675M) at 1/4000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 38 kD
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (HIF1AN) monoclonal Antibody, Unconjugated (SLM-51675M) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.Dylight® 554 Phalloidin (red) was used to stain the cell Cytoplasmic actin.
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