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Product Name ADK Chinese Name 腺苷酸激酶单克隆抗体 Alias Adenosine kinase; AK; EC:2.7.1.20; Adenosine 5'-phosphotransferase; ADK_HUMAN; Research Area Tumour Signal transduction The new supersedes the old Immunogen Species Mouse Clonality Monoclonal Clone NO. N3W5 React Species Human, Applications WB=1:500-4000 IHC-P=1:25 IHC-F=1:25 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 41kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen Recombinant human ADK between 1-345 amino acids. Lsotype IgG1,k Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
Function:
ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
Subunit:
Monomer.
Tissue Specificity:
Widely expressed. Highest level in placenta, liver, muscle and kidney.
DISEASE:
Hypermethioninemia due to adenosine kinase deficiency (HMAKD) [MIM:614300]: A metabolic disorder characterized by global developmental delay, early-onset seizures, mild dysmorphic features, and characteristic biochemical anomalies, including persistent hypermethioninemia with increased levels of S-adenosylmethionine and S-adenosylhomocysteine. Homocysteine levels are typically normal. Note=The disease is caused by mutations affecting the gene represented in this entry.
Similarity:
Belongs to the carbohydrate kinase PfkB family.
SWISS:
P55263
Gene ID:
132
Database links:Entrez Gene: 132 Human
SwissProt: P55263 Human
Product Picture Sample:
Lane 1: HepG2 cell lysates
Lane 2: HL-60 cell lysates
Lane 3: Human liver tissue lysates
Primary: Anti-ADK (SLM-51668M) at 1/4000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (human kidney tissue sections ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADK) Monoclonal Antibody, Unconjugated (SLM-51668M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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