Product Name	PDXK
Chinese Name	吡哆醛激酶单克隆抗体
Alias	PDXK.1; PDXK_HUMAN; PKH; PNK; Pyridoxal kinase; Pyridoxamine kinase; Pyridoxine kinase; Vitamin B6 kinase.
Research Area	Cell biology  Signal transduction  Epigenetics
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	H8F01
React Species	Human,
Applications	WB=1:500-4000 IHC-P=1:25 IHC-F=1:25 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	35kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human PDXK between 1-312 amino acids.
Lsotype	IgG1,k
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Pyridoxal kinase belongs to the pyridoxine kinase family and phosphorylates vitamin B6, a step necessary for the conversion of vitamin B6 to pyridoxal 5’ phosphate (PLP), the active form of vitamin B6. PLP acts as a coenzyme and functions to maintain homeostasis. Pyridoxal kinase is a 312-amino acid cytoplasmic protein that may act as a homodimer and is expressed ubiquitously. There are three known isoforms of pyridoxal kinase, and isoform 3 expression is observed in adult testis and spermatozoa. The optimum pH for pyridoxal kinase is between 5.5 and 6.0. PDXK, the gene that encodes the pyridoxal kinase protein, maps to chromosome 21q22.3 and may be a candidate gene for autoimmune polyglandular disease type 1, a genetic disorder that has been mapped to the same region on chromosome 21. Function: Required for synthesis of pyridoxal-5-phosphate from vitamin B6. Subunit: Homodimer (Probable). Subcellular Location: Cytoplasm. Tissue Specificity: Ubiquitous. Isoform 3 is detected in adult testis and spermatozoa. Similarity: Belongs to the pyridoxine kinase family. SWISS: O00764 Gene ID: 8566 Database links: Entrez Gene: 8566 Human SwissProt: O00764 Human
Product Picture	Sample: Lane 1: 293 cell lysates Lane 2: MCF-7 cell lysates Lane 3: U-251 MG cell lysates Lane 4: Human liver tissue lysates Primary: Anti-PDXK (SLM-51665M) at 1/4000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 35 kD Paraformaldehyde-fixed, paraffin embedded (human testis tissue sections); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PDXK) Monoclonal Antibody, Unconjugated (SLM-51665M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

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