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Product Name CAMK2 beta Chinese Name 钙/钙调素依赖蛋白激酶CAMK2B单克隆抗体 Alias Calcium/calmodulin dependent protein kinase (CaM kinase) II beta; Calcium/calmodulin dependent protein kinase II beta; Calcium/calmodulin dependent protein kinase IIB; Calcium/calmodulin dependent protein kinase type II beta chain; calcium/calmodulin-dependent protein kinase II beta; Calcium/calmodulin-dependent protein kinase type II subunit beta; CAM 2; CaM kinase II beta chain; CaM kinase II beta subunit; CaM kinase II subunit beta; CaM-kinase II beta chain; CAM2; CAMK 2; CAMK 2B; CaMK II beta subunit; CaMK II subunit beta; CaMK-II subunit beta; CAMK2; Camk2b; CAMKB; CaMKII beta subunit; CaMKIIB; KCC2B_HUMAN; MGC29528; Proline rich calmodulin dependent protein kinase; proline rich calmodulin-dependent protein kinase; Research Area Cell biology Neurobiology Signal transduction Cell type markers Epigenetics Immunogen Species Mouse Clonality Monoclonal Clone NO. F4A2 React Species Human, Mouse, (predicted: Rat, ) Applications WB=1:500-2000 IHC-P=1:25 IHC-F=1:25 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 61kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen Recombinant human CAMK2 between 1-503 amino acids. Lsotype IgG1 Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The multifunctional CaMKII beta, or Ca2+/calmodulin-dependent protein kinase II, is a well known effector of calcium- and calmodulin- mediated functions. It is present in many tissues but is most abundant in the brain. CaMKII is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Autophosphorylation plays an important role in the regulation of the kinase activity. CaMKII is required for synaptic plasticity, as in Long Term Potentiation (LTP), a cellular model for learning and memory.
Function:
Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in sarcoplamic reticulum Ca(2+) transport in skeletal muscle and may function in dendritic spine and synapse formation and neuronal plasticity. In slow-twitch muscles, is involved in regulation of sarcoplamic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of the ryanodine receptor-coupling factor triadin. In neurons, may participate in the promotion of dendritic spine and synapse formation and maintenance of synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning.
Subunit:
CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other.
Subcellular Location:
Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (Probable).
Tissue Specificity:
Expressed in skeletal muscle.
Post-translational modifications:
Autophosphorylation of Thr-287 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-287 locks the kinase into an activated state.
Similarity:
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
SWISS:
Q13554
Gene ID:
816
Database links:Entrez Gene: 816 Human
Entrez Gene: 12323 Mouse
SwissProt: Q13554 Human
SwissProt: P28652 Mouse
Product Picture Sample:
Lane 1: Jurkat cell lysates
Lane 2: PC-12 cell lysates
Lane 3: Human brain tissue lysates
Lane 4: Mouse Heart tissue lysates
Primary: Anti-CAMK2 beta (SLM-51658M) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 61 kD
Paraformaldehyde-fixed, paraffin embedded ( human cerebellum tissue sections ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CAMK2 beta) Monoclonal Antibody, Unconjugated (SLM-51658M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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