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Product Name GLS2 Chinese Name 谷氨酰胺酰胺水解酶GLS2单克隆抗体 Alias breast cell glutaminase; GLSL_HUMAN; glutaminase 2 (liver mitochondrial); glutaminase 2; glutaminase GA; glutaminase I; Glutaminase liver isoform; hLGA; L glutaminase; L glutamine amidohydrolase; L-glutaminase; L-glutamine amidohydrolase; LGA; mitochondrial; phosphate activated glutaminase; phosphate-dependent glutaminase. Research Area Tumour Cell biology Signal transduction The new supersedes the old Mitochondrion Immunogen Species Mouse Clonality Monoclonal Clone NO. Y09A1 React Species Human, Mouse, Applications WB=1:500-2000 IHC-P=1:50-200 IHC-F=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 65kDa Cellular localization cytoplasmic Mitochondrion Form Liquid Concentration 1mg/ml immunogen Recombinant human GLS2. Lsotype IgG1,k Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail LGA is a 602 amino acid protein that localizes to the mitochondrion and contains two ANK repeats. Expressed in brain, liver and pancreas, LGA functions as a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. LGA is overexpressed in breast cancer cell lines, suggesting a role for LGA in tumorigenesis. The gene encoding LGA maps to human chromosome 12, which encodes over 1,100 genes and comprises approximately 4.5% of the human genome. Chromosome 12 is associated with a variety of diseases and afflictions, including hypochondrogenesis, achondrogenesis, Kniest dysplasia, Noonan syndrome and Trisomy 12p, which causes facial developmental defects and seizure disorders.
Function:
Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
Subunit:
Interacts with the PDZ domain of the syntrophin SNTA1.Interacts with the PDZ domain of TAX1BP3.
Subcellular Location:
Mitochondrion.
Tissue Specificity:
Highly expressed in liver. Expressed in brain and pancreas. Not observed in heart, placenta, lung, skeletal muscle and kidney. Expression is significantly reduced in hepatocellular carcinomas.
Similarity:
Belongs to the glutaminase family.
Contains 2 ANK repeats.
SWISS:
Q9UI32
Gene ID:
27165
Database links:Entrez Gene: 27165 Human
Entrez Gene: 216456 Mouse
SwissProt: Q9UI32 Human
SwissProt: Q571F8 Mouse
Product Picture Sample:
Lane 1: Human brain tissue lysates
Lane 2: Mouse liver tissue lysates
Primary: Anti-GLS2 (SLM-51645M) at 1/1000~2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
Paraformaldehyde-fixed, paraffin embedded (Human kidney tissue sections); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLS2) Monoclonal Antibody, Unconjugated (SLM-51645M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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