Product Name	GLS2
Chinese Name	谷氨酰胺酰胺水解酶GLS2单克隆抗体
Alias	breast cell glutaminase; GLSL_HUMAN; glutaminase 2 (liver mitochondrial); glutaminase 2; glutaminase GA; glutaminase I; Glutaminase liver isoform; hLGA; L glutaminase; L glutamine amidohydrolase; L-glutaminase; L-glutamine amidohydrolase; LGA; mitochondrial; phosphate activated glutaminase; phosphate-dependent glutaminase.
Research Area	Tumour  Cell biology  Signal transduction  The new supersedes the old  Mitochondrion
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	Y09A1
React Species	Human, Mouse,
Applications	WB=1:500-2000 IHC-P=1:50-200 IHC-F=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	65kDa
Cellular localization	cytoplasmic Mitochondrion
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human GLS2.
Lsotype	IgG1,k
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	LGA is a 602 amino acid protein that localizes to the mitochondrion and contains two ANK repeats. Expressed in brain, liver and pancreas, LGA functions as a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. LGA is overexpressed in breast cancer cell lines, suggesting a role for LGA in tumorigenesis. The gene encoding LGA maps to human chromosome 12, which encodes over 1,100 genes and comprises approximately 4.5% of the human genome. Chromosome 12 is associated with a variety of diseases and afflictions, including hypochondrogenesis, achondrogenesis, Kniest dysplasia, Noonan syndrome and Trisomy 12p, which causes facial developmental defects and seizure disorders. Function: Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation. Subunit: Interacts with the PDZ domain of the syntrophin SNTA1.Interacts with the PDZ domain of TAX1BP3. Subcellular Location: Mitochondrion. Tissue Specificity: Highly expressed in liver. Expressed in brain and pancreas. Not observed in heart, placenta, lung, skeletal muscle and kidney. Expression is significantly reduced in hepatocellular carcinomas. Similarity: Belongs to the glutaminase family. Contains 2 ANK repeats. SWISS: Q9UI32 Gene ID: 27165 Database links: Entrez Gene: 27165 Human Entrez Gene: 216456 Mouse SwissProt: Q9UI32 Human SwissProt: Q571F8 Mouse
Product Picture	Sample: Lane 1: Human brain tissue lysates Lane 2: Mouse liver tissue lysates Primary: Anti-GLS2 (SLM-51645M) at 1/1000~2000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 65 kD Paraformaldehyde-fixed, paraffin embedded (Human kidney tissue sections); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLS2) Monoclonal Antibody, Unconjugated (SLM-51645M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

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