Product Name	SNAI1
Chinese Name	SNAIL单克隆抗体
Alias	SNAI1_HUMAN; Zinc finger protein SNAI1; SNAI1; SNAH; SNAI 1; SNA; SNAIL1; dJ710H13.1; Protein snail homolog 1 (Protein sna); Protein snail homolog 1; Protein sna;
Research Area	Tumour  Cardiovascular  Cell biology  Signal transduction  transcriptional regulatory factor  Zinc finger protein  Epigenetics
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	S1F6
React Species	Human,
Applications	WB=1:200-1000 ELISA=1:5000-10000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	29kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human SNAI1
Lsotype	IgMκ
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The zinc finger transcription factor 'SNAIL' was first identified in Drosophila and, along with 'twist', a basic helix-loop-helix transcription factor, is indispensable for mesoderm formation. SNAIL is a repressor of mouse E-cadherin transcription, with expression of SNAIL inversely correlated with expression of E-cadherin. Abnormal expression of SNAIL could underlie the tumorigenic conversion of epithelia associated with loss of E-cadherin expression through screening mouse and human cell lines and by in situ hybridization of primary human tumors undergoing malignant progression. Function: Involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm. Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. Subunit: Interacts with FBXL14 and GSK3B. Interacts with BTRC; interaction occurs when it is phosphorylated on the destruction motif. Interacts (via SNAG domain) with WTIP (via LIM domains). Interacts (via SNAG domain) with LIMD1 (via LIM domains), and AJUBA (via LIM domains). Interacts with LOXL2 and LOXL3. Subcellular Location: Nucleus. Cytoplasm. Note=Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs. Tissue Specificity: Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. Post-translational modifications: Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. O-GlcNAcylation at Ser-112 is enhanced in hyperglycaemic conditions, it opposes phosphorylation by GSK3B, and stabilizes the protein. Similarity: Belongs to the snail C2H2-type zinc-finger protein family. Contains 4 C2H2-type zinc fingers. SWISS: O95863 Gene ID: 6615 Database links: Entrez Gene: 6615 Human SwissProt: O95863 Human
Product Picture	Sample: Lane 1: Human HepG2 cell lysates Lane 2: Human HeLa cell lysates Lane 3: Human 293T cell lysates Lane 4: Human MDA-MB-231 cell lysates Lane 5: Human MCF-7 cell lysates Primary: Anti-SNAI1 (SLM-51463M) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa Sample: Lane 1: Hela cell lysate Lane 2: A549 cell lysate Lane 3: NCI-H460 cell lysate Primary: Anti-SNAIL (SLM-51463M) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 32 kD

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