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Mouse Anti-ATG12 antibody
Mouse Anti-ATG12 antibody
Apg12; APG12-like; APG12L; ATG12; ATG12 autophagy related 12 homolog; Autophagy 12; Autophagy-related protein 12; HAPG12; Ubiquitin-like protein ATG12; ATG12_HUMAN.
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Details

Product Name ATG12
Chinese Name 自噬相关蛋白12单克隆抗体
Alias Apg12; APG12-like; APG12L; ATG12; ATG12 autophagy related 12 homolog; Autophagy 12; Autophagy-related protein 12; HAPG12; Ubiquitin-like protein ATG12; ATG12_HUMAN.  
Research Area Tumour  Apoptosis  
Immunogen Species Mouse
Clonality Monoclonal
Clone NO. AT03
React Species (predicted: Human, )
Applications WB=1:500-2000 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 15/21kDa
Cellular localization cytoplasmic 
Form Liquid
Concentration 1mg/ml
immunogen Recombinant human APG12L. 
Lsotype IgG2b, Igk
Purification affinity purified by Protein G
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998[PubMed 9852036]).[supplied by OMIM].

Function:
Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Ubiquitous.

Similarity:
Belongs to the ATG12 family.

SWISS:
O94817

Gene ID:
9140

Database links:

Entrez Gene: 9140 Human

SwissProt: O94817 Human




Product Picture
Sample:
Lane 1: ATG12 Recombinant protein
Primary:
Anti-ATG12 (SLM-51462M) at 1/4000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 15/21 kD
Observed band size: 24 kD

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