Product Name	Myc Tag
Chinese Name	Myc tag标签单克隆抗体
Alias	avian myelocytomatosis viral oncogene homolog; bHLHe39; c-Myc; class E basic helix-loop-helix protein 39; MRTL; MYC; Myc Epitope Tag; myc proto-oncogene protein; myc-related translation/localization regulatory factor; oncogene c-Myc; proto-oncogene c-Myc; protooncogene homologous to myelocytomatosis virus; transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog (avian).
Product Type	Tag anti
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	9E10
React Species	Recombinant protein
Applications	WB=1:500-2000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Myc Tag
Lsotype	IgG1
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast,insect and mammalian cell systems. The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment. SWISS: P01106 Gene ID: 4609 Database links: Entrez Gene: 4609 Human Entrez Gene: 17869 Mouse Entrez Gene: 24577 Rat Omim: 190080 Human SwissProt: P01106 Human SwissProt: P01108 Mouse SwissProt: P09416 Rat Unigene: 202453 Human Unigene: 2444 Mouse Unigene: 12072 Rat
Product Picture	Sample: Lane1: Myc-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Lane2: Myc-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Primary: Anti-Myc tag (SLM-51003M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD Western blot analysis of 12-tag protein lysate, using Myc Tag Antibody(Cat. SLM-51003M). SLM-51003M was diluted at 1:2000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20ug. Anti-Myc Tag Antibody at 1:2000 dilution + 12 tag recombinant protein lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45-50 kDa

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