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Product Name SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Chinese Name SARS冠状病毒2核衣壳蛋白单克隆抗体(新冠抗原检测原料,可识别奥密克戎) Alias SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Protein; 2019 ncov N Protein; 2019-nCoV nucleocapsid protein; Ormicron mutant; Ormicron Nucleocapsid Protein; Ormicron N Protein. Research Area Bacteria and viruses Immunogen Species Rabbit Clonality Monoclonal Clone NO. 7B2 Applications WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 46kDa Form Liquid Concentration >1mg/ml immunogen Recombinant SARS-CoV-2 Nucleocapsid Protein Lsotype IgG Purification Affinity purified by Protein A Buffer Solution 0.01M PBS(pH7.4) Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
SWISS:
P0DTC9
Gene ID:
43740575
Product Picture Sample:
Lane 1:SARS-CoV-2 N Protein (WT) at 10ng
Lane 2:SARS-CoV-2 N Protein (Del204 & Del215) at 10ng
Lane 3:SARS-CoV-2 N Protein (R203M & D377Y) at 10ng
Primary: Rabbit Anti- SARS-CoV-2 N Protein Antibody at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:46kD
Observed band size:46kD
Sample:
Lane 1:SARS-CoV-2 N Protein (WT) at 10ng
Lane 2:SARS-CoV-2 N Protein (Del204 & Del215) at 2ng
Lane 3:SARS-CoV-2 N Protein (R203M & D377Y) at 2ng
Primary: Rabbit Anti- SARS-CoV-2 N Protein Antibody at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:46kD
Observed band size:46kD
Sample:
Lane 1: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 100ng
Lane 2: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 50ng
Lane 3: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 25ng
Lane 4: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 10ng
Lane 5: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 5ng
Lane 6: Recombinant SARS-CoV-2 N Protein(WT) at 10ng
Primary: Rabbit Anti- SARS-CoV-2 N protein (SLM-41511R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:45.3 kD
Observed band size:50 kD
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