Product Name	SARS-CoV-2 (2019-nCoV) Nucleocapsid
Chinese Name	SARS冠状病毒2核衣壳蛋白单克隆抗体
Alias	SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Protein; 2019 ncov N Protein; 2019-nCoV nucleocapsid protein.
literatures	Specific References  (2)     |     SLM-41412M has been referenced in 2 publications. [IF=8.116] Valeria Napolitano. et al. Acriflavine, a clinically approved drug, inhibits SARS-CoV-2 and other betacoronaviruses. Cell Chem Biol. 2022 Jan;:  IF ;  Human.   PubMed:35021060 [IF=4.123] Chunyan Zhang. et al. Foundation and Clinical Evaluation of a New Method for Detecting SARS-CoV-2 Antigen by Fluorescent Microsphere Immunochromatography. Front Cell Infect Mi. 2020; 10: 553837   PubMed:33330119
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	7A7
Applications	WB=1:500-2000 ELISA=1:5000-10000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	46kDa
Form	Liquid
Concentration	≥1 mg/ml
immunogen	Recombinant SARS-CoV-2 Nucleocapsid Protei: full length
Lsotype	IgG2b
Purification	affinity purified by Protein G
Buffer Solution	0.01M PBS(pH7.4) with 0.1% Proclin300
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. SWISS: P0DTC9 Gene ID: 43740575
Product Picture	Sample: Lane 1: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 100ng Lane 2: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 50ng Lane 3: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 25ng Lane 4: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 10ng Lane 5: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 5ng Lane 6: Recombinant SARS-CoV-2 N Protein(WT) at 10ng Primary: Mouse Anti- SARS-CoV-2 N protein (SLM-41412M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size:45.3 kD Observed band size:50 kD Sample: Lane 1: SARS-CoV-2 N protein(Cat# SL41408P) at 50ng Lane 2: SARS-CoV-2 N protein(Cat# SL41408P) at 25ng Lane 3: SARS-CoV-2 N protein(Cat# SL41408P) at 5ng Lane 4: SARS-CoV-2 N protein(Cat# SL41408P) at 2ng Lane 5: SARS-CoV-2 N protein(Cat# SL41408P) at 1ng Lane 6:SARS-CoV-2 N protein(Cat# SL41408P) at 0.5ng Primary: Anti- SARS-CoV-2 N protein (SL41412M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD Sample: Lane 1: Recombinant SARS-CoV-2 N protein (WT) (His Tag) (SL41408P) Lane 2: Recombinant SARS-CoV-2 N protein (Q9H, P67S, P80R, P151L, S183Y) (His Tag) (SL41451P) Lane 3: Recombinant SARS-CoV-2 N protein (D3L, P13T, D103Y, D128Y, H145Y, R203K, G204R, T205I, S235F) (His Tag) (SL41452P) Lane 4: Recombinant SARS-CoV-2 N protein (Del204, Del215) (His Tag) (SL41491P) Lane 5: Recombinant SARS-Cov-2 N protein (R203M, D377Y) (His Tag) (SL41492P) Lane 6: Recombinant SARS-Cov-2 (Omicron, B.1.1.529) N protein (P13L, E31del, R32del, S33del, R203K, G204R) (N-His Tag) (SL41494P) Primary: Anti-SARS-CoV-2(2019-nCoV)Nucleocapsid(SLM-41412M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 50 kDa

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