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Product Name Gamma Tubulin Chinese Name 微管蛋白γ单克隆抗体 Alias Centrosome Marker; Gamma 1 tubulin; gamma tubulin; gamma-tubulin; Gamma 2 tubulin; Gamma Tubulin 1; Gamma Tubulin 2; Gamma tubulin complex component 1; GCP 1; GCP-1; GCP1; MGC131994; TUBG; TUBG1; TUBG2; Tubulin gamma 1 chain; Tubulin gamma 2 chain; Xgam; TBG1_HUMAN; TBG2_HUMAN; Tubulin gamma-1 chain; Gamma-1-tubulin; Gamma-tubulin complex component 1. Research Area Cell biology Neurobiology Cytoskeleton Immunogen Species Mouse Clonality Monoclonal Clone NO. 8D11 React Species Human, Mouse, (predicted: Rat, ) Applications WB=1:500-5000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 50kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Gamma Tubulin Lsotype IgG Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Centrosome Marker
Gamma tubulin, a member of the tubulin superfamily, is a ubiquitous and highly conserved protein within the microtubule organizing centre (MTOC). Gamma tubulin is not a component of microtubules, rather it functions as the microtubule nucleator at the MOTC, is responsible for binding microtubule minus ends and mediating the link between microtubles and the centrosome. By binding to the beta tubulin subunit of the tubulin molecule, it establishes the polarity of a microtubule leaving the alpha tubulin subunit exposed at the positive end.
The abundance of Gamma tubulin is less than 1% of the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton.
Function:
Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
Subunit:
Interacts with TUBGCP2 and TUBGCP3. Interacts with B9D2 (By similarity). Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Interacts with PIFO.
Subcellular Location:
Cytoplasm, cytoskeleton, centrosome.
Tissue Specificity:
Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
Post-translational modifications:
Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
Similarity:
Belongs to the tubulin family.
SWISS:
P23258
Gene ID:
7283
Database links:Entrez Gene: 27175 Human
Entrez Gene: 7283 Human
Entrez Gene: 103733 Mouse
Entrez Gene: 103768 Mouse
Omim: 191135 Human
Omim: 605785 Human
SwissProt: P23258 Human
SwissProt: Q9NRH3 Human
SwissProt: P83887 Mouse
SwissProt: Q8VCK3 Mouse
Unigene: 279669 Human
Unigene: 708059 Human
Unigene: 142348 Mouse
Unigene: 479145 Mouse
Unigene: 154431 Rat
结构蛋白(Structural Proteins)
gamma tubulin 微管蛋白是存于中心体的另一种微管蛋白, gamma微管蛋白对微管的形成具有重要作用。gamma微管蛋白通过与β-微管蛋白的相互作用帮助微管的成核反应(nucleation)。即在微管的组装中gamma微管蛋白先形成一个圆或形成钩环结构, γ微管蛋白的这种结构可指导微管蛋白二聚体结合上去并进行微管的组装。gamma微管蛋白在微管的组装中起关键作用。Product Picture Sample:
Lane 1: Mouse NIH/3T3 cell lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Rat Cerebellum tissue lysates
Lane 4: Human HeLa cell lysates
Lane 5: Human Jurkat cell lysates
Lane 6: Human HepG2 cell lysates
Lane 7: Human A549 cell lysates
Primary: Anti-Gamma Tubulin (SLM-33313M) at 1/3000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 46 kDa
Sample:
A549(Human) Cell Lysate at 30 ug
MCF-7(Human) Cell Lysate at 30 ug
U87MG(Human) Cell Lysate at 30 ug
Primary: Anti- Gamma Tubulin (SLM-33313M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma Tubulin) Polyclonal Antibody, Unconjugated (SLM-33313M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma Tubulin) Polyclonal Antibody, Unconjugated (SLM-33313M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma Tubulin) Monoclonal Antibody, Unconjugated (ascites of SLM-33313M-8D11) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma Tubulin) Monoclonal Antibody, Unconjugated (ascites of SLM-33313M 8D11) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma Tubulin) Monoclonal Antibody, Unconjugated (ascites of SLM-33313M 8D11) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma Tubulin) Monoclonal Antibody, Unconjugated (ascites of SLM-33313M 8D11) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.Blank control(black line):Hela.
Primary Antibody (green line):Mouse Anti-Gamma Tubulin antibody (SLM-33313M)
Dilution:1ug/Test;
Secondary Antibody(white blue line): Goat anti-Mouse IgG-AF488
Dilution: 0.5ug/Test.
Isotype control(orange line): Normal Mouse IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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