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Product Name FUT4 Chinese Name α-(1,3)-岩藻糖基转移酶 4单克隆抗体 Alias SSEA-1; 3-FAL; Alpha (1,3) fucosyltransferase; Alpha 13 fucosyltransferase FucT; EC 2.4.1.; ELAM 1 ligand fucosyltransferase; ELAM ligand fucosyltransferase; ELAM1 ligand fucosyltransferase; ELFT; FCT3A; Fuc TIV; Fucosyltransferase 4 alpha 1 3 fucosyltransferase myeloid specific; Fucosyltransferase 4; FucT IV; FUCTIV; Galactoside 3 L fucosyltransferase; Lewis X; LeX; SSEA 1; SSEA1; Stage specific embryonic antigen 1; FUC-TIV. Research Area Tumour Cell biology immunology The cell membrane受体 Immunogen Species Mouse Clonality Monoclonal Clone NO. 8B9 React Species Human, Applications IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 58kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human FUT4 Lsotype IgG Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq].
Function:
May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.
Subcellular Location:
Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi.
Tissue Specificity:
Highest expression in stomach and colon. It is also expressed in the lung, testis, uterus, small intestine and to a lesser extent in spleen, and ovary. Present in trace amounts in brain, thymus, heart, smooth muscle, kidney and bone marrow. Not found in liver, salivary gland and pancreas.
Similarity:
Belongs to the glycosyltransferase 10 family.
SWISS:
P22083
Gene ID:
2526
Database links:Entrez Gene: 10690 Human
Entrez Gene: 2526 Human
Entrez Gene: 2527 Human
Entrez Gene: 2528 Human
Entrez Gene: 2529 Human
Omim: 104230 Human
SwissProt: P22083 Human
SwissProt: P51993 Human
SwissProt: Q11128 Human
SwissProt: Q11130 Human
SwissProt: Q9Y231 Human
Unigene: 390420 Human
Unigene: 457 Human
Unigene: 49117 Human
Unigene: 572064 Human
Unigene: 623098 Human
Unigene: 631843 Human
Unigene: 631846 Human
Unigene: 705615 Human
FUT4,也称为 ELFT 和 FCT3A,属于糖基转移酶 10 家族。FUT4 可催化参与 Lewis X/SSEA-1 和 VIM-2 抗原表达的 alpha-1,3 糖苷键。FUT4 是一种抗原表位,定义为 Lewis X 碳水化合物结构,在鼠胚胎癌细胞 (EC)、鼠 ES 和 iPS 细胞以及鼠和人类生殖细胞上表达。它被广泛用作小鼠未分化 ES 和 iPS 细胞的阳性表面Maker和人未分化 ES 和 iPS 细胞的阴性表面Maker。Product Picture Paraformaldehyde-fixed, paraffin embedded (Human esophageal); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FUT4) Monoclonal Antibody, Unconjugated (ascites of SLM-33111M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (human tonsil tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FUT4) Monoclonal Antibody, Unconjugated (ascites of SLM-33111M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
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