Product Name	CK18
Chinese Name	细胞角蛋白18单克隆抗体
Alias	K1C18_HUMAN; Keratin, type I cytoskeletal 18; KRT18; CYK18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18 (CK-18); Keratin-18 (K18); PIG46; Keratin 18;
literatures	Specific References  (2)     |     SLM-33101M has been referenced in 2 publications. [IF=5.279] Qiushuang Sun. et al. Regulation on Citrate Influx and Metabolism through Inhibiting SLC13A5 and ACLY: A Novel Mechanism Mediating the Therapeutic Effects of Curcumin on NAFLD. J Agr Food Chem. 2021;XXXX(XXX):XXX-XXX  WB ;  Mouse.   PubMed:34323067 [IF=4.275] Xiuyun Xu. et al. Sox9+ cells are required for salivary gland regeneration after radiation damage via the Wnt/β-catenin pathway. J Genet Genomics. 2021 Oct;:  IF ;  Mouse.   PubMed:34757039
Research Area	Tumour  Cell biology  immunology  Signal transduction
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	3C3
React Species	Human, Rat,  (predicted: Mouse, )
Applications	WB=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	48kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human CK18
Lsotype	IgG
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] Function: When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. Post-translational modifications: Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-231 by either caspase-3, caspas-6 or caspase-7. O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residue. Similarity: Belongs to the intermediate filament family. SWISS: P05783 Gene ID: 3875 Database links: Entrez Gene: 506480 Cow Entrez Gene: 3875 Human Entrez Gene: 16668 Mouse Entrez Gene: 294853 Rat Omim: 148070 Human SwissProt: P05783 Human SwissProt: P05784 Mouse SwissProt: Q5BJY9 Rat Unigene: 406013 Human Unigene: 22479 Mouse Unigene: 103924 Rat
Product Picture	Sample: A431 Cell (Human) Lysate at 40 ug Primary: Anti- CK18 (SLM-33101M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD Sample: MCF-7 Cell (Human) Lysate at 40 ug HepG2 Cell (Human) Lysate at 40 ug Primary: Anti- CK18 (SLM-33101M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD Sample: Lane 1: Small intestine (Rat) Lysate at 40 ug Lane 2: Pancreas (Rat) Lysate at 40 ug Lane 3: Lung (Rat) Lysate at 40 ug Lane 4: Uterus (Rat) Lysate at 40 ug Primary: Anti-CK18 (SLM-33101M ) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 45 kD Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK18) Monoclonal Antibody, Unconjugated (SLM-33101M) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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