Product Name	RFP
Chinese Name	红色荧光蛋白单克隆抗体
Alias	Red fluorescent protein; Red fluorescent protein eqFP611; RFPTag; RFP-Tag; RFP Tag; RFP_ENTQU.
Product Type	Tag anti
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	7A5
React Species	Recombinant protein
Applications	WB=1:1000-5000 ELISA=1:1000-5000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant Red fluorescent protein
Lsotype	IgG
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.Red Fluorescent Protein (RFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm. Function: Pigment protein. Post-translational modifications: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-(N+2), oxidation of Tyr-(N+1) to didehydrotyrosine, and formation of a double bond to the alpha-amino nitrogen of residue Xaa-N. Maturation of the chromophore requires nothing other than molecular oxygen. SWISS: Q8ISF8 Gene ID: N/A
Product Picture	Sample: Lane 1: RFP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41403P) at 2ug Lane 2: RFP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41403P) at 4ug Primary: Anti-RFP-Tag (SLM-33011M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD Sample: Lane 1: RFP-Tagged fusion protein (full length) Overexpression E.coli Lysate (Cat#: SL33011P) at 4 ug Primary: Anti-RFP-Tag (SLM-33011M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 26 kD

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