Product Name	human CD33-APC
Chinese Name	APC标记人CD33单克隆抗体
Alias	CD33_HUMAN; CD 33; CD33 antigen; CD33 molecule; FLJ00391; gp67 ; Myeloid cell surface antigen CD33; Myeloid cell surface antigen CD33 precursor; p67 ; Sialic acid binding Ig like lectin 3; Sialic acid binding immunoglobulin like lectin 3; SIGLEC3; Siglec-3; myeloid cell surface antigen CD33 isoform 1 precursor.  APC anti-human CD33;
Research Area	immunology  Stem cells  Cell Surface Molecule
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	HI33a
React Species	Human,
Applications	Flow-Cyt=5ul/Test  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	40/37kDa
Cellular localization	The cell membrane
Form	Liquid
Lsotype	IgG2a
Purification	affinity purified by Protein G
Buffer Solution	0.01M PBS, 0.5%BSA, 0.03%Proclin300
Storage	Store at 2-8℃ for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias. Function: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Subunit: Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Monocytic/myeloid lineage cells. Post-translational modifications: Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. Similarity: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: P20138 Gene ID: 945 Database links: Entrez Gene: 945 Human Omim: 159590 Human SwissProt: P20138 Human Unigene: 83731 Human
Product Picture	Flow cytometry staining of normal human peripheral blood cells with CD33/APC(SLM-30078M-APC)(Red histogram) or Mouse IgG2a Isotype Control (APC Conjugate)(C03-12004)(Blue histogram) . Total cells were used for analysis. Blank control:U-937. Primary Antibody (green line): Rabbit Anti-CD33 antibody (SLM-30078M-APC) Dilution: 1ug/Test; Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.

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