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Product Name AFP(A2) Chinese Name 甲胎蛋白单克隆抗体(包被) Alias Alpha Fetoprotein; Alpha-fetoprotein; alpha 1 fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; alpha fetoglobulin; alpha Fetoprotein; Alpha fetoprotein precursor; FETA; HPAFP; FETA_HUMAN. literatures Specific References (12) | SLM-1621M has been referenced in 12 publications.Research Area Tumour Immunogen Species Mouse Clonality Monoclonal Clone NO. A2 React Species Human, Rat, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 65kDa Cellular localization Secretory protein Form Liquid Concentration 1mg/ml immunogen AFP protein: full length Lsotype IgG Purification affinity purified by Protein G Buffer Solution 0.01M PBS(pH7.4) with 0.03% Proclin300. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.
Subunit:
Dimeric and trimeric forms have been found in addition to the monomeric form.
Subcellular Location:
Secreted.
Tissue Specificity:
Plasma. Synthesized by the fetal liver and yolk sac.
Similarity:
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.
SWISS:
P02771
Gene ID:
174
Database links:Entrez Gene: 174 Human
Omim: 104150 Human
SwissProt: P02771 Human
Unigene: 518808 Human
Product Picture Sample: AFP recombinded protein lysate at 100ng;
Primary: Anti-AFP(A4) (SLM-1622M) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-mouse IgG(SL0296G-HRP) at 1: 5000 dilution;
Predicted band size: 65 kD
Observed band size: 65 kDSample:
Lane 1: Rat Embryo Lysates
Lane 2: Rat Placenta Lysates
Primary: Anti-AFP(A2) (SLM-1621M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 65kDa
Observed band size: 65kDa
Sample: AFP recombinded protein lysate at 100ng; Primary: Anti-AFP(A4) (SLM-1622M) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-mouse IgG(SL0296G-HRP) at 1: 5000 dilution; Predicted band size: 65 kD Observed band size: 65 kDParaformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AFP(A2)) Polyclonal Antibody, Unconjugated (SLM-1621M) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-cy3) for 90 minutes, and DAPI for nuclei staining.
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