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Mouse Anti-AFp (A2)antibody
Mouse Anti-AFp (A2)antibody
Alpha Fetoprotein; Alpha-fetoprotein; alpha 1 fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; alpha fetoglobulin; alpha Fetoprotein; Alpha fetoprotein precursor; FETA; HPAFP; FETA_HUMAN.
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  • NO.:SLM-1621M
    Clonality:Monoclonal
    Immunogen Species:Mouse
    React Species:Human,Rat,
    Applications:WB ELISA IHC-P IHC-F IF
    concentration:1mg/ml
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Details

Product Name AFP(A2)
Chinese Name 甲胎蛋白单克隆抗体(包被)
Alias Alpha Fetoprotein; Alpha-fetoprotein; alpha 1 fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; alpha fetoglobulin; alpha Fetoprotein; Alpha fetoprotein precursor; FETA; HPAFP; FETA_HUMAN.  
literatures
Specific References  (12)     |     SLM-1621M has been referenced in 12 publications.
[IF=10.05] Aoxiang Xiao. et al. Ultrasensitive Detection and Cellular Photothermal Therapy via a Self-Photothermal Modulation Biosensor. ADV OPT MATER. 2023 Jan;:2202711  Rat.  
[IF=7.48] Zong, Chen, et al. "Multilayer hemin/G-quadruplex wrapped gold nanoparticles as tag for ultrasensitive multiplex immunoassay by chemiluminescence imaging." Biosensors and Bioelectronics 43 (2013): 372-378.  ELISA ;  
[IF=6.45] Lin, Dajie, et al. "Cascade signal amplification for electrochemical immunosensing by integrating biobarcode probes, surface-initiated enzymatic polymerization and silver nanoparticle deposition." Biosensors and Bioelectronics (2014).  other ;  
[IF=6.408] Chellachamy Anbalagan, Amarnath. et al. Redox-labelled detection probe enabled immunoassay for simultaneous detection of multiple cancer biomarkers. MICROCHIM ACTA. 2023 Mar;190(3):1-9  
[IF=6.277] Zhao CR et al. Highly sensitive and selective electrochemical immunosensors by substrate-enhanced electroless deposition of metal nanoparticles onto three-dimensional Graphene@Ni foams. Science Bulletin.2019.  Other ;  
[IF=6.042] Chen Z et al. Chemical Redox-Cycling for Improving the Sensitivity of Colorimetric ELISA.(2018) Anal. Chem.  ELISA ;  Human.  
[IF=5.88] Zhang, Xin, et al. "Spectrum-Based Electrochemiluminescent Immunoassay with Ternary CdZnSe Nanocrystals as Labels." Analytical Chemistry (2016).  
[IF=5.83] Zong, Chen, et al. "Chemiluminescence imaging immunoassay of multiple tumor markers for cancer screening." Analytical Chemistry 84.5 (2012): 2410-2415.  other ;  Human.  
[IF=5.123] Song X et al. Multiplex immunoassays using surface modification-mediated porous layer open tubular capillary.Anal Chim Acta. 2018 Dec 28;1043:1-10.  LIF detection&ELISA ;  Human.  
[IF=4.52] Zhao, Changrong, et al. "Multiplexed electrochemical immunoassay using streptavidin/nanogold/carbon nanohorn as a signal tag to induce silver deposition." Analytica Chimica Acta (2014).  other ;  
[IF=3.998] A. Ikeda. et al. Follistatin expressed in mechanically-damaged salivary glands of male mice induces proliferation of CD49f + cells. Sci Rep-Uk. 2020 Nov;10(1):1-14  ICC ;  Mouse.  
[IF=2.475] Huang Y et al. Ag@Au Core⁻Shell Porous Nanocages with Outstanding SERS Activity for Highly Sensitive SERS Immunoassay. Sensors (Basel). 2019 Mar 31;19(7). pii: E1554.  ELISA ;  
Research Area Tumour  
Immunogen Species Mouse
Clonality Monoclonal
Clone NO. A2
React Species Human, Rat, 
Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 65kDa
Cellular localization Secretory protein 
Form Liquid
Concentration 1mg/ml
immunogen AFP protein: full length 
Lsotype IgG
Purification affinity purified by Protein G
Buffer Solution 0.01M PBS(pH7.4) with 0.03% Proclin300.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.

Subunit:
Dimeric and trimeric forms have been found in addition to the monomeric form.

Subcellular Location:
Secreted.

Tissue Specificity:
Plasma. Synthesized by the fetal liver and yolk sac.

Similarity:
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.

SWISS:
P02771

Gene ID:
174

Database links:

Entrez Gene: 174 Human

Omim: 104150 Human

SwissProt: P02771 Human

Unigene: 518808 Human



Product Picture
Sample: AFP recombinded protein lysate at 100ng;
Primary: Anti-AFP(A4) (SLM-1622M) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-mouse IgG(SL0296G-HRP) at 1: 5000 dilution;
Predicted band size: 65 kD
Observed band size: 65 kD
Sample:
Lane 1: Rat Embryo Lysates
Lane 2: Rat Placenta Lysates
Primary: Anti-AFP(A2) (SLM-1621M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 65kDa
Observed band size: 65kDa
Sample: AFP recombinded protein lysate at 100ng; Primary: Anti-AFP(A4) (SLM-1622M) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-mouse IgG(SL0296G-HRP) at 1: 5000 dilution; Predicted band size: 65 kD Observed band size: 65 kD
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AFP(A2)) Polyclonal Antibody, Unconjugated (SLM-1621M) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-cy3) for 90 minutes, and DAPI for nuclei staining.
Measured by its binding ability in a indirect ELISA. Immobilized Human AFP protein, His Tag (Cat. SL43140P) at 2 μg/mL (100 μL/well) can bind   Mouse Anti-Human AFP Antibody, the EC50 is 24.94 ng/mL.

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