Product Name	His tag(2D5)
Chinese Name	聚组氨酸单克隆抗体
Alias	HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.
literatures	Specific References  (19)     |     SLM-0287M has been referenced in 19 publications. [IF=5.29] Li, Mo-fei, Jun Li, and Li Sun. "CsMAP34, a teleost MAP with dual role: A promoter of MASP-assisted complement activation and a regulator of immune cell activity." Scientific Reports 6 (2016): 39287.  IF(ICC) ;   PubMed:28008939 [IF=4.407] Yu Zhang. et al. An adeno-associated virus-mediated immunotherapy for Alzheimer’s disease. Mol Immunol. 2022 Apr;144:26  WB,IHC ;  Human,Mouse.   PubMed:10.1016/j.molimm.2022.02.006 [IF=4.39] Qin, Jialin, et al. "Selective and sensitive homogenous assay of serum albumin with 1-anilinonaphthalene-8-sulphonate as a biosensor." Analytica Chimica Acta (2014).  other ;   PubMed:24856404 [IF=4.33] Wang, Yan, et al. "Enhanced tolerance and remediation to mixed contaminates of PCBs and 2, 4-DCP by transgenic alfalfa plants expressing the 2, 3-dihydroxybiphenyl-1, 2-dioxygenase." Journal of Hazardous Materials (2014).  WB ;   PubMed:25590820 [IF=4.07] Sun, Yun, et al. "Edwardsiella tarda Eta1, an In Vivo-Induced Antigen That Is Involved in Host Infection." Infection and immunity 80.8 (2012): 2948-2955.   PubMed:22585967 [IF=4.02] Sun et al. Pseudomonas fluorescens Filamentous Hemagglutinin, an Iron-Regulated Protein, Is an Important Virulence Factor that Modulates Bacterial Pathogenicity. (2016) Front.Microbiol. 7:1320  IF ;   PubMed:27602029 [IF=3.895] Kumar V et al. PirABVP Toxin Binds to Epithelial Cells of the Digestive Tract and Produce Pathognomonic AHPND Lesions in Germ-Free Brine Shrimp. Toxins (Basel). 2019 Dec 9;11(12). pii: E717.  IHC-P ;   PubMed:31835437 [IF=3.7] Zhou, Ze-jun, and Li Sun. "CsCTL1, a teleost c-type lectin that promotes antibacterial and antiviral immune defense in a manner that depends on the conserved EPN motif." Developmental & Comparative Immunology (2015).   PubMed:25636784 [IF=3.62] Gao, Lili, et al. "The role of a novel C-type lectin-like protein from planarian in innate immunity and regeneration." Developmental & Comparative Immunology (2016).  WB ;   PubMed:27565408 [IF=3.53] Zhang J, Zhang Bc, Sun L (2015) P247 and P523: Two In Vivo-Expressed Megalocytivirus Proteins That Induce Protective Immunity and Are Essential to Viral Infection. PLoS ONE 10(3): e0121282   PubMed:25815484 [IF=3.24] Zhang, Bao-cun, et al. "Rock bream (< i> Oplegnathus fasciatus) viperin is a virus-responsive protein that modulates innate immunity and promotes resistance against megalocytivirus infection." Developmental & Comparative Immunology (2014).   PubMed:24525178 [IF=2.96] Yu, Lan-ping, et al. "Characterization of a c-type lysozyme of Scophthalmus maximus: expression, activity, and antibacterial effect." Fish & Shellfish Immunology (2012).   PubMed:23063540 [IF=2.96] Li, Mo-Fei, et al. "A peptidoglycan recognition protein from< i> Sciaenops ocellatus is a zinc amidase and a bactericide with a substrate range limited to Gram-positive bacteria." Fish & Shellfish Immunology (2011).   PubMed:22146700 [IF=2.82] Li, Mo-fei, and Yong-hua Hu. "C5a of Cynoglossus semilaevis has anaphylatoxin-like properties and promotes antibacterial and antiviral defense." Developmental & Comparative Immunology (2016).   PubMed:26934108 [IF=2.82] Zhang, Bao-cun, and Li Sun. "Tongue sole (Cynoglossus semilaevis) prothymosin alpha: cytokine-like activities associated with the intact protein and the C-terminal region that lead to antiviral immunity via Myd88-dependent and–independent pathways respectively." Developmental & Comparative Immunology (2015).   PubMed:26162512 [IF=2.82] Sun, Yuan-yuan, Li Liu, and Jun Li. "Three novel B-type mannose-specific lectins of Cynoglossus semilaevis possess varied antibacterial activities against Gram-negative and Gram-positive bacteria." Developmental & Comparative Immunology (2015).  ELISA ;   PubMed:26455466 [IF=2.82] Li, Mo-fei, and Jian Zhang. "CsTNF1, a teleost tumor necrosis factor that promotes antibacterial and antiviral immune defense in a manner that depends on the conserved receptor binding site." Developmental & Comparative Immunology (2015).   PubMed:26478190 [IF=2.67] Sun, Qing-lei, and Li Sun. "A short-type peptidoglycan recognition protein from tongue sole (Cynoglossus semilaevis) promotes phagocytosis and defense against bacterial infection." Fish & Shellfish Immunology (2015).   PubMed:26364742 [IF=0] Wang, S. M., et al. "A novel nanobody specific for respiratory surfactant protein A has potential for lung targeting." Clinical, Cosmetic and Investigational Dermatology 8 (2015): 193-204.  IHC-F ;   PubMed:25914462
Product Type	Tag anti
Research Area	Cell biology  immunology
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	2D5
React Species	Recombinant protein
Applications	WB=1:5000-50000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated HHHHHH peptide: HHHHHH
Lsotype	IgG
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. SWISS: N/A Gene ID: N/A 蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白 1） 6xHis与不同的Sepharose纯化填料结合（捆绑），可不受构象的影响，在天然或变形的条件下可进行进一步纯化。 2） 可在温和的洗脱液环境中，其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。 3） 6xHis标签比普通（Tag）要小得多，经纯化后的蛋白可直接进行下游操作。可用于任何表达系统（包括： 细菌，杆状病毒和哺乳动物） 4） 6xHis标签在生理溶液pH下不带电荷，其标签不会干涉重组蛋白的结构和功能， 6xHis不会影响蛋白分泌。 5） 6xHis标签免疫原性差，重组蛋白可以不必除去标签而直接作为抗原进行免疫。
Product Picture	Sample: His tag protein (Human) at 100 ng His tag protein (Human) at 100 ng His tag protein (Human) at 100 ng Primary: Anti- His tag(2D5) (SLM-0287M) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 35 kD Sample: Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41403P) at 2ug Lane 2: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41403P) at 4ug Primary: Anti-His-tag (SLM-0287M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD Sample: Lane1: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41230P) at 2ug Lane2: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41230P) at 2ug Primary: Anti-His tag (SLM-0287M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD

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