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Product Name INTS3 Chinese Name 集成复杂蛋白亚单位3抗体 Alias Integrator complex subunit 3; C1orf193; C1orf60; FLJ21919; INT 3; INT3; INTS3; SOSS complex subunit A. Research Area Cell biology Signal transduction Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Dog, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 118kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human INTS3: 901-1043/1043 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail RNA polymerase II (Pol II) is an enzyme that is composed of twelve subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The integrator complex is a multi-protein complex that associates with the C-terminal domain of Pol II and is involved in small nuclear RNA (snRNA) transcription and 3’-end processing. INTS3 (integrator complex subunit 3), also known as INT3 or SOSS-A, is a 1,043 amino acid nuclear protein and is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit and mediates 3-prime end processing of small nuclear RNAs U1 and U2. Expressed in the nucleus, INTS3 exists as four alternatively spliced isoforms and is encoded by a gene located on human chromosome 1q21.3.
Subunit:
Belongs to the multiprotein complex Integrator, at least composed of INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8, INTS9/RC74, INTS10, CPSF3L/INTS11 and INTS12. Component of the SOSS complex, composed of SOSS-B (SOSS-B1/OBFC2B or SOSS-B2/OBFC2A), SOSS-A/INTS3 and SOSS-C/C9orf80. SOSS complexes containing SOSS-B1/OBFC2B are more abundant than complexes containing SOSS-B2/OBFC2A. Interacts with SOSS-B1/OBFC2B, SOSS-B2/OBFC2A and SOSS-C/C9orf80; the interaction is direct. Interacts with NBN/NBS1.
Subcellular Location:
Nucleus. Note=Localizes to nuclear foci following DNA damage.
Similarity:
Belongs to the Integrator subunit 3 family.
SWISS:
Q68E01
Gene ID:
65123
Database links:Entrez Gene: 65123 Human
Entrez Gene: 229543 Mouse
Omim: 611347 Human
SwissProt: Q68E01 Human
SwissProt: Q7TPD0 Mouse
Product Picture Sample:
Hela(Human) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
Primary: Anti- INTS3 (SL9766R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 118 kD
Observed band size: 118 kD
Paraformaldehyde-fixed, paraffin embedded (Human uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (INTS3) Polyclonal Antibody, Unconjugated (SL9766R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (INTS3) Polyclonal Antibody, Unconjugated (SL9766R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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