Product Name	phospho-STMN1 (Ser63)
Chinese Name	磷酸化原癌基因蛋白18抗体
Alias	Stathmin 1 (phospho S63); p-Stathmin 1 (phospho S63); Stathmin 1 (Phospho-Ser63); Stathmin (Phospho Ser63); Stathmin (Phospho S63); Lag; LAP 18; LAP18; Leukemia associated phosphoprotein p18; Metablastin; Oncoprotein 18; OP 18; OP18; p18; p19; Phosphoprotein 19; Phosphoprotein p19; PP17; PP19; PR22; Pr22 protein; Prosolin; Protein Pr22; SMN; Stathmin; Stathmin1; STMN 1; STMN1_HUMAN.
Product Type	Phosphorylated anti
Research Area	Tumour  Cell biology  Neurobiology  Signal transduction  Cell adhesion molecule  Cytoskeleton
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Cow, Rabbit, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	16kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthesised phosphopeptide derived from human Stathmin 1 around the phosphorylation site of Ser63: 41-140/149
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009] Function: Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear. Subunit: Binds to two alpha/beta-tubulin heterodimers. Interacts with KIST. Subcellular Location: Cytoplasm, cytoskeleton. Tissue Specificity: Ubiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia. Post-translational modifications: Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization. Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity. Similarity: Belongs to the stathmin family. SWISS: P16949 Gene ID: 3925 Database links: Entrez Gene: 3925 Human Entrez Gene: 16765 Mouse Entrez Gene: 29332 Rat Omim: 151442 Human SwissProt: P16949 Human SwissProt: P54227 Mouse SwissProt: P13668 Rat Unigene: 209983 Human Unigene: 378957 Mouse Unigene: 555 Rat 原癌基因蛋白18(oncogene protein 18,Op18),是一种广泛存在于细胞质的蛋白质,可与微管蛋白结合,参与微管和纺锤体的组装;与细胞的增殖、分化、再生和运动均有关,并具有信号活性调节的功能。在细胞增殖、分化的信号途径、传到通路激活中也具有多种调节功能。 近年来有研究发现,stathmin在多种Tumour中高表达,并可通过调节微管的解聚,促进Tumour细胞的运动及侵袭。Stathmin翻译后修饰状态的改变,可影响与p53蛋白的相互作用,参与Tumour的发生发展.
Product Picture	Paraformaldehyde-fixed, paraffin embedded (mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-STMN1 (Ser63)) Polyclonal Antibody, Unconjugated (SL9764R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-STMN1 (Ser63)) Polyclonal Antibody, Unconjugated (SL9764R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (STMN1) Polyclonal Antibody, Unconjugated (SL9764R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (STMN1) Polyclonal Antibody, Unconjugated (SL9764R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (STMN1) Polyclonal Antibody, Unconjugated (SL9764R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Positive control: (mo)Thymocyte (2% Paraformaldehyde-fixed ) Isotype Control Antibody: Rabbit IgG; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary Antibody: rabbit Anti-phospho-Stathmin 1 (Ser63) (SL9764R); Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA

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