TEL: +86 571 56623320 EMAIL: [email protected]
Product Name TNIP1 Chinese Name Tumour坏死因子诱导蛋白3相互作用蛋白1抗体 Alias ABIN 1; ABIN-1; abin1; HIV-1 Nef interacting protein (Nip40-1); TNFAIP3 interacting protein 1; HIV-1 Nef interacting protein; HIV-1 Nef-interacting protein; hVAN; KIAA0113; NAF 1; NAF-1; NAF1; Nef-associated factor 1; Nef-associated factor 1 SNP; Nip 40-1; Nip40-1; TNFAIP3 interacting protein 1; TNFAIP3-interacting protein 1; TNIP 1; TNIP-1; Tnip1; TNIP1_HUMAN; VAN; Virion associated nuclear shuttling protein; Virion-associated nuclear shuttling protein. literatures Specific References (1) | SL9568R has been referenced in 1 publications.[IF=1.813] Zhou Xueling. et al. A20-Binding Inhibitor of NF-κB 1 Ameliorates Neuroinflammation and Mediates Antineuroinflammatory Effect of Electroacupuncture in Cerebral Ischemia/Reperfusion Rats. Evid-Based Compl Alt. 2020;2020:6980398 IF ; Rat.Research Area Developmental biology Chromatin and nuclear signals Signal transduction transcriptional regulatory factor lymphocyte Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Dog, Pig, Cow, Horse, Rabbit, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 72kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human TNIP1: 351-450/636 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Abin-1 is a 636 amino acid protein that localizes to both the nucleus and the cytoplasm and is shuttled between the two intercellular regions in a CRM1-dependent manner. Expressed ubiquitously with highest expression in spleen and skeletal muscle, Abin-1 interacts with A20 and, via this interaction, interferes with TRAF2-mediated transactivation signals and effectively inhibits TNF-induced NFⅹB expression. Additionally, Abin-1 can be incorporated into HIV-1 virions and, if overexpressed, can inhibit viral replication. Abin-1 may also play an important role in the regulation of nuclear import and export activities. Multiple isoforms of Abin-1 exist due to alternative splicing events.
Function:
Interacts with zinc finger protein A20/TNFAIP3 and inhibits TNF-induced NF-kappa-B-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal (By similarity). Increases cell surface CD4(T4) antigen expression. Interacts with HIV-1 matrix protein and is packaged into virions and overexpression can inhibit viral replication. May regulate matrix nuclear localization, both nuclear import of PIC (Preintegration complex) and export of GAG polyprotein and viral genomic RNA during virion production.
Subunit:
Interacts with TNFAIP3 and IKBKG; facilitates TNFAIP3-mediated de-ubiquitination of NEMO/IKBKG. Interacts with HIV-1 matrix protein. Interacts with Shigella flexneri ipah9.8; the interaction promotes polyubiquitination of IKBKG. Interacts with polyubiquitin. Interacts with MAPK1, SELPLG and PIK3CD. Interacts with IKBKG (polyubiquitinated). Interacts with IRAK1 (polyubiquitinated). Interacts with MYD88; the interaction is indicative for participation in an activated TLR-signaling complex
Subcellular Location:
Cytoplasm. Nucleus. Note=Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner.
Tissue Specificity:
Ubiquitous. Strongly expressed in peripheral blood lymphocytes, spleen and skeletal muscle, and is weakly expressed in the brain.
Post-translational modifications:
Phosphorylation at Tyr-552 by SRC-family kinases recruits phosphoinositide-3-kinase (PI3K) PIK3CD:p85 heterodimer which results in integrin activation and leukocyte adhesion to activated endothelium during inflammation.
Similarity:
Cytoplasm. Nucleus. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner.
SWISS:
Q15025
Gene ID:
10318
Database links:Entrez Gene: 10318 Human
Omim: 607714 Human
SwissProt: Q15025 Human
Unigene: 355141 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNIP1) Polyclonal Antibody, Unconjugated (SL9568R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TNIP1 Polyclonal Antibody, Unconjugated(SL9568R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Scan Wechat Qrcode
Scan Whatsapp Qrcode