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Product Name SLC2A4RG Chinese Name 葡萄糖Transporter4增强因子抗体 Alias GEF; GLUT4 enhancer factor; HDBP 1; HDBP-1; HDBP1; Huntington disease gene regulatory region binding protein 1; Huntington disease gene regulatory region-binding protein 1; S2A4R_HUMAN; Si 1 2 19; Si 1 2; SLC2A4 regulator; SLC2A4RG; Zinc finger protein 2 5 Glut4 enhancer factor. Research Area Cell biology Signal transduction transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Cow, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 41kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SLC2A4RG: 301-387/387 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is a nuclear transcription factor involved in the activation of the solute carrier family 2 member 4 gene. The encoded protein interacts with another transcription factor, myocyte enhancer factor 2, to activate transcription of this gene. [provided by RefSeq, Jul 2008].
Function:
Transcription factor involved in SLC2A4 and HD gene transactivation. Binds to the consensus sequence 5'-GCCGGCG-3'.
Subunit:
Interacts with MEF2A.
Subcellular Location:
Cytoplasm. Nucleus. Note=Shuttles between the cytoplasm and the nucleus.
Tissue Specificity:
According to PubMed:14630949, expressed in heart, skeletal muscle, liver, kidney and pancreas; undetectable in lung, placenta or brain. According to PubMed:14625278, ubiquitously expressed, with lowest expression in brain and ileum.
Similarity:
Contains 1 C2H2-type zinc finger.
SWISS:
Q9NR83
Gene ID:
56731
Database links:Entrez Gene: 56731 Human
Omim: 609493 Human
SwissProt: Q9NR83 Human
Unigene: 435126 Human
Product Picture Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Mouse Muscle tissue lysates
Primary: Anti-SLC2A4RG (SL9457R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti- SLC2A4RG (SL9457R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SLC2A4RG) Polyclonal Antibody, Unconjugated (SL9457R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SLC2A4RG) Polyclonal Antibody, Unconjugated (SL9457R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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