Product Name	OIF
Chinese Name	骨诱导因子抗体
Alias	Corneal keratan sulfate proteoglycan; MIME_HUMAN; mimecan; Mimecan proteoglycan; OG; OGN; OIF; osteoglycin; osteoinductive factor; SLRR3A.
Research Area	Cell biology  Signal transduction  Cell differentiation  Cytoskeleton  Extracellular matrix
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Rat,  (predicted: Human, Mouse, Pig, Cow, Horse, Rabbit, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	31kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human OIF: 101-200/298
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The small leucine-rich proteoglycan (SLRP) family of proteins contains various proteins such as Decorin, Biglycan, Fibromodulin, Keratocan, Lumican, Osteoadherin and Osteoglycin. These proteins all have similar functions as they all mediate extracellular matrix organization and act as binding partners of TGF beta. Osteoglycin, which also may be designated osteoinductive factor (OIF), is a secreted protein detected in bone tissues. Osteoglycin induces the formation of bone in conjunction with either TGF-beta-1 or TGF-beta-2. The precursor form of the OGN gene product, designated Mimecan, is subject to in situ proteolytic cleavage to yield the mature Osteoglycin. Function: Induces bone formation in conjunction with TGF-beta-1 or TGF-beta-2. Subcellular Location: Secreted, extracellular space, extracellular matrix. Tissue Specificity: Bone. Post-translational modifications: N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. Similarity: Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class III subfamily. Contains 7 LRR (leucine-rich) repeats. SWISS: P20774 Gene ID: 4969 Database links: Entrez Gene: 4969 Human Omim: 602383 Human SwissProt: P20774 Human Unigene: 109439 Human
Product Picture	Tissue/cell: rat bone marrow; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-OIF Polyclonal Antibody, Unconjugated(SL9407R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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