Product Name	CT135/ODF3
Chinese Name	Tumour/睾丸抗原135抗体
Alias	Cancer/testis antigen 135; CT135; hSHIPPO; odf3; ODF3A_HUMAN; Outer dense fiber of sperm tails 3; Outer dense fiber of sperm tails protein 3; Outer dense fiber protein 3; SHIPPO1; Sperm tail protein SHIPPO 1; Sperm tail protein SHIPPO1; TISP50; Transcript induced in spermiogenesis protein 50.
Research Area	Tumour  Cell biology  Developmental biology  Cyclin  Cell differentiation
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	(predicted: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	28kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ODF3/CT135: 101-200/254
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Outer dense fibers are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail. May help to maintain the passive elastic structures and elastic recoil of the sperm tail. Constituting the main cytoskeletal structure of spermatid flagella, outer dense fibers (ODFs) add elastic recoil, stiffness and protection against shear forces during sperm movement. Human ODFs consist of approximately 10 major and at least 15 minor proteins. The major proteins of the ODF include Odf1, Odf2, and Odf3, which compose a family of proteins that are preferentially expressed during mammalian spermiogenesis. Odf3 (outer dense fiber protein 3), also known as sperm tail protein SHIPPO 1 and TISP50 (transcript induced in spermiogenesis protein 50), is a 254 amino acid protein that is expressed during the latter part of spermatogenesis in flagella of elongated spermatids and mature sperm. Odf proteins are directed to their exact subcellular location by Spags, which are characterized as chaperone-like Odf-binding molecules. There are two isoforms of Odf3 that are produced as a result of alternative splicing events. Function: Outer dense fibers are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail. May help to maintain the passive elastic structures and elastic recoil of the sperm tail. Subcellular Location: Cytoplasm. Expressed in the cytoplasmic lobe of spermatids. Tissue Specificity: Testis-specific. Similarity: Belongs to the ODF3 family. Contains 2 STPGR (Sperm-tail PG-rich) repeats. SWISS: Q96PU9 Gene ID: 113746 Database links: Entrez Gene: 113746 Human Entrez Gene: 69287 Mouse Omim: 608356 Human SwissProt: Q96PU9 Human SwissProt: Q920N1 Mouse Unigene: 350949 Human

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