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Product Name BTBD1 Chinese Name BTB/POZ结构域蛋白1抗体 Alias BTB (POZ) domain containing 1; BTB domain containing 1; BTB/POZ domain containing protein 1; BTBD 1; BTBD1; C15orf1; HCV NS5A transactivated protein 8; Hepatitis C virus NS5A transactivated protein 8; NS5ATP8; BTBD1_HUMAN. Research Area Cell biology Cyclin transcriptional regulatory factor Bacteria and viruses Cell differentiation Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Horse, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 53kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human BTBD1: 101-200/482 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail BTBD1 contains a proline-rich region and a BTB/POZ domain which are typically involved in protein-protein interactions. The C-terminus of BTBD1 binds topoisomerase I and BTBD1 also interacts with TRIM5 isoform Delta. BTBD1 localizes to cytoplasmic bodies and is essential for myogenic differentiation.
Function:
Probable substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Subunit:
Interacts with topoisomerase 1 and with TRIM5 isoform Delta. Interacts with CUL3.
Subcellular Location:
Cytoplasm, P-body. Cytoplasmic bodies.
Tissue Specificity:
Ubiquitous; higher levels in heart and skeletal muscle.
Similarity:
Contains 1 BTB (POZ) domain.
SWISS:
Q9BSF8
Gene ID:
84280
Database links:Entrez Gene: 84280 Human
Entrez Gene: 68815 Mouse
SwissProt: Q9BSF8 Human
SwissProt: Q80X66 Mouse
Product Picture Sample: Testis (Mouse) Lysate at 40 ug
Primary: Anti-BTBD1 (SL9294R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-BTBD1 (SL9294R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BTBD1) Polyclonal Antibody, Unconjugated (SL9294R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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