Product Name	IGSF21
Chinese Name	免疫球蛋白超家族成员21抗体
Alias	FLJ41177; IGSF 21; Immunoglobin superfamily member 21; immunoglobulin superfamily member 21; MGC15730; RP11 121A23.1; IGS21_HUMAN.
Research Area	Tumour  Cell biology  immunology  Neurobiology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Rat,  (predicted: Human, Mouse, Chicken, Dog, Pig, Cow, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	49kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human IGSF21: 81-180/467
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	IGSF21 is a 467 amino acid secreted protein that contains two Ig-like (immunoglobulin-like) domains and is a member of the immunoglobulin superfamily. Members of this family of proteins usually localize to the cell membrane, and may act as receptors in the immune response pathways. The gene encoding IGSF21 maps to human chromosome 1p36.13 and mouse chromosome 4 D3. Human chromosome 1 spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8% of the human genome. A large number of disease-associated genes are associated with chromosome 1, including those that are involved in familial adenomatous polyposis, Stickler syndrome, Parkinson’s disease, Gaucher disease, schizophrenia and Usher syndrome. Aberrations in chromosome 1 are found in a variety of cancers, including head and neck cancer, malignant melanoma and multiple myeloma. Subcellular Location: Secreted Similarity: Contains 2 Ig-like (immunoglobulin-like) domains. SWISS: Q96ID5 Gene ID: 84966 Database links: Entrez Gene: 84966 Human SwissProt: Q96ID5 Human Unigene: 212511 Human
Product Picture	Sample: Cerebellum (mouse) Lysate at 40 ug Primary: Anti- IGSF21(SL9204R) at 1/200 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution Predicted band size: 49 kD Observed band size: 49/69 kD Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IGSF21) Polyclonal Antibody, Unconjugated (SL9204R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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