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Product Name GHRHR Chinese Name 生长激素释放因子受体抗体 Alias GHRFR; GHRH R; GHRH receptor; GHRHR_HUMAN; GHRHRpsv; GRF R; GRF receptor; GRFR; Growth hormone releasing factor receptor; Growth hormone releasing hormone receptor; Growth hormone-releasing factor receptor; Growth hormone-releasing hormone receptor. Research Area Cell biology Neurobiology Signal transduction Growth factors and hormones G protein-coupled receptor G protein signal Immunogen Species Rabbit Clonality Polyclonal React Species Human, Rat, (predicted: Mouse, Dog, Pig, Cow, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 45kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from Rabbit GHRHR: 341-423/423 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail GHRH-R is a seven transmembrane domain protein that localizes to the somatotroph of the pituitary. GHRH-R plays an important role in growth and acts as a high-affinity receptor for GHRH. Binding of GHRH leads to the coupling of GHRH-R to G-protein which stimulates increased adenylyl cyclase activity and the accumulation of cAMP leading to the synthesis and release of growth hormone and somatotroph proliferation. In addition, this signalling pathway may have direct action in fetal/placental development, reproduction and immune function. GHRH and GHRH-R may also play a role in the regulation of non-rapid eye movement sleep (NREMS). The expression of GHRH-R is dependent on the presence of the POU domain factor Pit-1. Mutations in the gene encoding this protein can result in isolated growth hormone deficiency (IGHD), also known as Dwarfism of Sindh, and anterior pituitary hypoplasia (APH).
Function:
Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion.
Subcellular Location:
Cell membrane.
Tissue Specificity:
Pituitary gland.
DISEASE:
Defects in GHRHR are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH.
Similarity:
Belongs to the G-protein coupled receptor 2 family.
SWISS:
Q02643
Gene ID:
2692
Database links:Entrez Gene: 2692 Human
Entrez Gene: 14602 Mouse
Omim: 139191 Human
SwissProt: Q02643 Human
SwissProt: P32082 Mouse
Unigene: 767 Human
Unigene: 89928 Mouse
Unigene: 10936 Rat
Product Picture Sample:
Adrenal gland (rat) Lysate at 40 ug
panc-1 (human)cell Lysate at 40 ug
Primary: Anti-GHRHR(SL8867R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 50 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GHRHR Polyclonal Antibody, Unconjugated(SL8867R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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