Product Name	SMAD8
Chinese Name	SMAD8蛋白抗体
Alias	AI528653 ; BSP 1 ; BSP1 ; DKFZp781C1895 ; DKFZp781O1323 ; Dwarfin A ; Dwf A ; DwfA ; Dwfc ; hSMAD1 ; hSmad5 ; JV 41 ; JV4 1 ; JV41 ; JV5 1 ; MAD (mothers against decapentaplegic, Drosophila) homolog 1 ; MAD (mothers against decapentaplegic, Drosophila) homolog 9 ; MAD homolog 1 ; MAD homolog 9 ; MAD homolog1 (mothers against decapentaplegic, Drosophila) ; Mad related protein 1 ; MAD, mothers against decapentaplegic homolog 9 ; MADH 1 ; Madh1 ; MADH5 ; MADH6 ; MADH9 ; Madr 1 ; MADR1 ; Mlp1 ; Mothers against decapentaplegic, drosophila, homolog of, 9 ; Mothers against DPP homolog 1 ; Mothers against DPP homolog 9 ; MusMLP ; OTTHUMP00000178565 ; OTTMUSP00000022970 ; OTTMUSP00000022971 ; OTTMUSP00000022972 ; OTTMUSP0000002324 ; OTTMUSP00000023428 ; Sma and Mad related protein 1 ; Sma- and Mad-related protein 1 ; SMAD ; SMAD family member 5 ; SMAD mothers against DPP homolog 1 Drosophila ; SMAD8 ; SMAD8A ; SMAD8B ; Spinal muscular atrophy distal with upper limb predominance ; TGF beta signaling protein 1 ; Transforming growth factor beta signaling protein 1 ; Transforming growth factor-beta signaling protein 1.
literatures	Specific References  (2)     |     SL8844R has been referenced in 2 publications. [IF=5.195] Weifeng Du. et al. Research on the effect of Dipsaci Radix before and after salt-processed on kidney yang deficiency syndrome rats and the preliminary mechanism study through the BMP-Smad signaling pathway. J ETHNOPHARMACOL. 2023 Aug;312:116480  IHC,WB ;  Rat.   PubMed:37061069 [IF=3.296] Li YT et al. Involvement of bone morphogenetic protein–related pathways in the effect of aucubin on the promotion of osteoblast differentiation in MG63 cells. Chemico-Biological Interactions, 283, 51–58.Chemico-Biological Interactions, 283, 51–58.  WB ;  Human.   PubMed:10.1016/j.cbi.2018.02.005
Research Area	Tumour  Cell biology  Neurobiology  Signal transduction  Growth factors and hormones  transcriptional regulatory factor  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Zebrafish, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	53kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human SMAD8: 351-450/467
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008] Function: Sphingomyelin synthases synthesize sphingolipids through transfer of a phosphatidyl head group on to the primary hydroxyl of ceramide. SAMD8 is an endoplasmic reticulum (ER) transferase that has no sphingomyelin synthase activity but can convert phosphatidylethanolamine (PE) and ceramide to ceramide phosphoethanolamine (CPE) albeit with low product yield. Appears to operate as a ceramide sensor to control ceramide homeostasis in the endoplasmic reticulum rather than a converter of ceramides. Seems to be critical for the integrity of the early secretory pathway Subcellular Location: Cytoplasmic and Nuclear. Similarity: Belongs to the sphingomyelin synthase family. Contains 1 SAM (sterile alpha motif) domain. SWISS: Q96LT4 Gene ID: 142891 Database links: Entrez Gene: 4086 Human Entrez Gene: 4090 Human Entrez Gene: 4093 Human Entrez Gene: 17125 Mouse Entrez Gene: 17129 Mouse Entrez Gene: 55994 Mouse Entrez Gene: 25671 Rat Entrez Gene: 59328 Rat Entrez Gene: 85435 Rat Omim: 601595 Human Omim: 603110 Human Omim: 603295 Human SwissProt: O15198 Human SwissProt: Q15797 Human SwissProt: Q99717 Human SwissProt: P70340 Mouse SwissProt: P97454 Mouse SwissProt: Q9JIW5 Mouse SwissProt: O54835 Rat SwissProt: P97588 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SMAD8) Polyclonal Antibody, Unconjugated (SL8844R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SMAD8) Polyclonal Antibody, Unconjugated (SL8844R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SMAD8) Polyclonal Antibody, Unconjugated (SL8844R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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