Product Name	phospho-GNA12 (Ser40)
Chinese Name	磷酸化鸟苷酸调节蛋白12抗体
Alias	GNA12(phospho-Ser40); GNA12(phospho Ser40); GNA12(phospho S40); P-GNA12(phospho S40); G alpha 12; gep; GNA12; Guanine nucleotide binding protein alpha 12 subunit; NNX3; RMP; AI414047; AI504261; ALPHA SUBUNIT OF THE G12 FAMILY OF HETEROTRIMERIC GS; FLJ21929; G PROTEIN ALPHA 12; MGC104623; MGC130498; MGC99644; GNA12_HUMAN.
Product Type	Phosphorylated anti
Research Area	Tumour  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Cow, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human GNA12: RR(p-S)RD
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail
Product Picture	Sample: DU145(Human) Cell Lysate at 30 ug Primary: Anti-phospho-GNA12(Ser40) (SL8812R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 44 kD Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GNA12(Ser40)) Polyclonal Antibody, Unconjugated (SL8812R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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