Product Name	SLC25A11
Chinese Name	溶质载体家族25成员11抗体
Alias	M2OM_HUMAN; Mitochondrial 2 oxoglutarate/malate carrier protein; Mitochondrial 2-oxoglutarate/malate carrier protein; OGC; OGCP; SLC20A4; Solute carrier family 20 (oxoglutarate carrier) member 4; Solute carrier family 20 member 4; Solute carrier family 25 (mitochondrial carrier oxoglutarate carrier) member 11; Solute carrier family 25 member 11.
Research Area	Tumour  Cell biology  Signal transduction  Transporter
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Cow, Rabbit, Sheep, Guinea Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1ug/Test  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	34kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human SLC25A15: 101-200/314
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The oxoglutarate/malate carrier transports 2-oxoglutarate across the inner membranes of mitochondria in an electroneutral exchange for malate or other dicarboxylic acids (summary by Iacobazzi et al., 1992 [PubMed 1457818]).[supplied by OMIM, Jan 2011] Function: Catalyzes the transport of 2-oxoglutarate across the inner mitochondrial membrane in an electroneutral exchange for malate or other dicarboxylic acids, and plays an important role in several metabolic processes, including the malate-aspartate shuttle, the oxoglutarate/isocitrate shuttle, in gluconeogenesis from lactate, and in nitrogen metabolism. Subcellular Location: Mitochondrion inner membrane. Similarity: Belongs to the mitochondrial carrier family. Contains 3 Solcar repeats. SWISS: Q02978 Gene ID: 8402 Database links: Entrez Gene: 8402 Human Entrez Gene: 67863 Mouse Entrez Gene: 64201 Rat Omim: 604165 Human SwissProt: Q02978 Human SwissProt: Q9CR62 Mouse SwissProt: P97700 Rat Unigene: 184877 Human Unigene: 296082 Mouse Unigene: 466994 Mouse Unigene: 853 Rat
Product Picture	Sample: Lane 1: Human U251 cell lysates Lane 2: Human A549 cell lysates Primary: Anti-SLC25A11 (SL8703R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 32 kDa Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-SLC25A15 (SL8703R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 34 kD Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-SLC25A15 antibody (SL8703R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST methanol for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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