Product Name	POD1
Chinese Name	足细胞表达蛋白/转录因子21抗体
Alias	bHLHa23; Capsulin; Class A basic helix-loop-helix protein 23; Epicardin; POD 1; Pod-1; POD1; Podocyte expressed 1; Podocyte-expressed 1; TCF-21; TCF21; tcf21 ; TCF21_HUMAN; Transcription factor 21.
literatures	Specific References  (1)     |     SL8688R has been referenced in 1 publications. [IF=4.271] A. Tran-Guzman. et al. Toxicity Profiles and Protective Effects of Antifreeze Proteins From Insect in Mammalian Models. TOXICOL LETT. 2022 Jul;:  IF ;  Rat.   PubMed:35901986
Research Area	Cardiovascular  Cell biology  Signal transduction  transcriptional regulatory factor  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	20 kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human TCF-21: 41-140/179
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The basic helix-loop-helix (bHLH) class of transcription factors govern cell fate determination by controlling a variety of cellular differentiation processes. POD-1 (podocyte-expressed 1, also designated capsulin or epicardin) is a nuclear bHLH protein that is involved in the specification of select mesodermal cell populations associated with heart, cranial skeletal muscle, gut and urogenital system. POD-1 is selectively expressed in mesenchymal cells at sites of epithelial-mesenchymal interaction in the kidney, lung, intestine, pancreas and the epicardium, which gives rise to the coronary arteries. This epithelial-mesenchymal interaction is involved in the formation of numerous internal organs. POD-1 is also expressed in the mesothelium that gives rise to the spleen and in cells that give rise to smooth muscle. In addition to its role in kidney morphogenesis and spleen organogenesis, POD-1 may play a role in the development and sex determination of the mammalian gonad. Function: Involved in epithelial-mesenchymal interactions in kidney and lung morphogenesis that include epithelial differentiation and branching morphogenesis. May play a role in the specification or differentiation of one or more subsets of epicardial cell types. Subunit: Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with TCF3 and binds the E box (5'-CANNTG-3'). Subcellular Location: Nucleus. Similarity: Contains 1 basic helix-loop-helix (bHLH) domain. SWISS: O43680 Gene ID: 6943 Database links: Entrez Gene: 421697 Chicken Entrez Gene: 510873 Cow Entrez Gene: 6943 Human Entrez Gene: 21412 Mouse Entrez Gene: 252856 Rat Omim: 603306 Human SwissProt: Q5E9S3 Cow SwissProt: O43680 Human SwissProt: O35437 Mouse Unigene: 78061 Human Unigene: 16497 Mouse
Product Picture	Sample: Cerebrum (Mouse) Lysate at 40 ug Kidney (Rat) Lysate at 40 ug Primary: Anti-POD1 (SL8688R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kD Observed band size: 23 kD Sample: K562(Human) Cell Lysate at 30 ug Primary: Anti-POD1 (SL8688R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kD Observed band size: 23 kD Tissue/cell: Rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti- POD1 Polyclonal Antibody, Unconjugated(SL8688R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat tongue tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-POD1 Polyclonal Antibody, Unconjugated(SL8688R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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