Product Name	IRE1
Chinese Name	内质网核Signal transduction蛋白a1抗体
Alias	IRE1a; Endoplasmic reticulum (ER) to nucleus signalling 1; Endoplasmic reticulum to nucleus signaling 1; Endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signaling 1; ERN 1; ERN1; ERN1_HUMAN; hIRE 1p; hIRE1p; Inositol requiring 1; Inositol requiring protein 1; Inositol-requiring protein 1; IRE-1; IRE 1; IRE 1a; IRE 1P; Ire1 alpha; Ire1-alpha; Ire1alpha; IRE1P; MGC163277; Protein kinase/endoribonuclease; Serine/threonine protein kinase/endoribonuclease IRE1.
literatures	Specific References  (21)     |     SL8680R has been referenced in 21 publications. [IF=12.153] Zhu, Zhenglin. et al. The natural product salicin alleviates osteoarthritis progression by binding to IRE1α and inhibiting endoplasmic reticulum stress through the IRE1α-IκBα-p65 signaling pathway. EXP MOL MED. 2022 Nov;:1-13  WB ;  Rat.   PubMed:36357568 [IF=6.614] Yiwei Yao. et al. CARMA3 deficiency aggravates angiotensin II-induced abdominal aortic aneurysms development interacting between endoplasmic reticulum and mitochondria. CAN J CARDIOL. 2023 Apr;:  IF,WB ;  Mouse.   PubMed:37030515 [IF=5.793] Chunyue Wang. et al. Neuroprotective effects of verbascoside against Alzheimer’s disease via the relief of endoplasmic reticulum stress in Aβ-exposed U251 cells and APP/PS1 mice. J Neuroinflamm. 2020 Dec;17(1):1-16  WB ;  Human.   PubMed:33070776 [IF=5.075] Angelica D. Reyes-Perea. et al. The Mycotoxin De-Epoxy-Deoxynivalenol (DOM-1) Increases Endoplasmic Reticulum Stress in Ovarian Theca Cells. TOXINS. 2023 Mar;15(3):228  WB ;  Bovine.   PubMed:36977119 [IF=4.868] Cui J et al. Acetaldehyde Induces Neurotoxicity In Vitro via Oxidative Stress-and Ca2. Oxid Med Cell Longev. 2019 Jan 9;2019:2593742.  WB ;  Mouse.   PubMed:30728884 [IF=4.86] Jiang, Mingfang, et al. "Down-regulation of miR-384-5p Attenuates Rotenone-induced Neurotoxicity in Dopaminergic SH-SY5Y Cells Through Inhibiting Endoplasmic Reticulum Stress." American Journal of Physiology-Cell Physiology (2016): ajpcell-00226.  WB ;  Human.   PubMed:26864693 [IF=4.294] Mei Zhang. et al. Fine particulate matter induces endoplasmic reticulum stress-mediated apoptosis in human SH-SY5Y cells. Neurotoxicology. 2022 Jan;88:187  WB ;  Human.   PubMed:34813867 [IF=4.19] Xu, Demei, et al. "Polychlorinated biphenyl quinone induces endoplasmic reticulum stress, unfolded protein response and calcium release." Chemical Research in Toxicology (2015).  WB ;  Human.   PubMed:25950987 [IF=4.07] He, Wencheng, et al. "CSFV Infection Up-Regulates the Unfolded Protein Response to Promote Its Replication." Frontiers in Microbiology 8 (2017): 2129.  WB ;  Pig.   PubMed:29163417 [IF=3.858] Li,et al.Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function.(2018) Toxicology Letters. 285:156-167.  WB ;  Human.   PubMed:29306025 [IF=3.457] Lu, Y. et al. Protective effects of Danzhi jiangtang capsule on vascular endothelial damages induced by high-fat diet and palmitic acid. (2018) Biomedicine & Pharmacotherapy, 107, 1631–1640.  WB ;  Rat.   PubMed:30257381 [IF=3.29] Yan, Jiting, et al. "Catalpol prevents alteration of cholesterol homeostasis in non-alcoholic fatty liver disease via attenuating endoplasmic reticulum stress and NOX4 over-expression." RSC Advances 7.2 (2017): 1161-1176.  WB ;  Human.   PubMed:10.1039/c6ra26046b [IF=3.28] Chen, Li, et al. "Influence of resveratrol on endoplasmic reticulum stress and expression of adipokines in adipose tissues/adipocytes induced by high-calorie diet or palmitic acid." Endocrine (2016): 1-13.  WB ;  Mouse.   PubMed:28070709 [IF=3.12] Jiang, H-Q., et al. "Guanabenz Delays the Onset of Disease Symptoms, Extends Lifespan, Improves Motor Performance and Attenuates Motor Neuron Loss in the SOD1 G93A Mouse Model of Amyotrophic Lateral Sclerosis." Neuroscience (2014).  WB ;  Mouse.   PubMed:24699224 [IF=3.038] Jiajia Chenet al. Novel Role of HAX-1 in Neurons Protection After Spinal Cord Injury Involvement of IRE-1. Neurochem Res . 2020 Oct;45(10):2302-2311.  WB, IHC ;  rat.   PubMed:32681444 [IF=2.791] Yang S et al. Induction of the unfolded protein response (UPR) during pseudorabies virus infection. Vet Microbiol. 2019 Dec;239:108485.  WB ;  Pig.   PubMed:31767094 [IF=2.776] He Q et al. Titanium dioxide nanoparticles induce mouse hippocampal neuron apoptosis via oxidative stress- and calcium imbalance-mediated endoplasmic reticulum stress. Environ Toxicol Pharmacol. 2018 Oct;63:6-15.  WB ;  Mouse.   PubMed:30114659 [IF=2.638] Yu-Dong Wei. et al. Dmrt1 regulates the immune response by repressing the TLR4 signaling pathway in goat male germline stem cells. Zool Res. 2021 Jan 18; 42(1): 14–27  WB ;  Goat.   PubMed:33420764 [IF=2.147] Gurur Garip. et al. Effect of endoplasmic reticulum stress on human trophoblast cells: Survival triggering or catastrophe resulting in death. ACTA HISTOCHEM. 2022 Oct;124:151951  IF ;  Human.   PubMed:35998395 [IF=2.092] Zhang Hao. et al. Protective effects of pterostilbene against hepatic damage, redox imbalance, mitochondrial dysfunction, and endoplasmic reticulum stress in weanling piglets. J Anim Sci. 2020 Oct;98(10):  WB ;  Pig.   PubMed:33027517 [IF=1.664] Kishi,et al.Pro-metastatic signaling of the trans fatty acid elaidic acid is associated with lipid rafts.(2018) Oncology Letters. 15:4423-4426.  WB ;  Mouse.   PubMed:29556289
Research Area	Cell biology  Signal transduction  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/test ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	105kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human IRE1a: 252-260/977
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq, Jul 2008] Function: Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. Subunit: Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds HSPA5, a negative regulator of the unfolded protein response. This interaction may disrupt homodimerization and prevent activation of ERN1. Interacts with TAOK3 and TRAF2. Subcellular Location: Endoplasmic reticulum membrane. Tissue Specificity: Ubiquitously expressed. High levels observed in pancreatic tissue. Post-translational modifications: Autophosphorylated. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 KEN domain. Contains 1 protein kinase domain. SWISS: O75460 Gene ID: 2081 Database links: Entrez Gene: 2081 Human Entrez Gene: 78943 Mouse Entrez Gene: 498013 Rat Omim: 604033 Human SwissProt: O75460 Human SwissProt: Q9EQY0 Mouse Unigene: 133982 Human Unigene: 592041 Human Unigene: 700027 Human Unigene: 20452 Mouse Unigene: 340943 Mouse Unigene: 226435 Rat
Product Picture	Sample: Lane 1: Human Raji cell lysates Lane 2: Human HeLa cell lysates Primary: Anti-IRE1 (SL8680R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 105 kDa Observed band size: 135 kDa Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IRE1) Polyclonal Antibody, Unconjugated (SL8680R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control: Raji. Primary Antibody (green line): Rabbit Anti-IRE1 antibody (SL8680R ) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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