Product Name	TYMS
Chinese Name	胸苷酸合成酶抗体
Alias	Thymidylate synthase; EC 2.1.1.45; HsT422; MGC88736; TS; TSase; OK/SW-cl.29; TYSY_HUMAN.
Research Area	Tumour  Cell biology  immunology  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Pig, Cow, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	36kDa
Cellular localization	The nucleus cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human TYMS: 65-160/313
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy. [provided by RefSeq, Aug 2017] Function: Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Subunit: Homodimer. Subcellular Location: Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane. Similarity: Belongs to the thymidylate synthase family. SWISS: P04818 Gene ID: 7298 Database links: Entrez Gene: 7298 Human Entrez Gene: 22171 Mouse Entrez Gene: 29261 Rat SwissProt: P04818 Human SwissProt: P07607 Mouse SwissProt: P45352 Rat 胸苷酸合成酶(thymidylate synthase,TS)是生物体内催化胸苷酸合成所必需的酶,多年来一直作为Tumour化疗的重要靶酶。对TS基因调控机制的研究表明:基因扩增、转录、翻译和翻译后过程都参与了TS表达的调控。TS可与自身的mRNA结合形成TS-mRNA复合物,使mRNA翻译受阻,5-氟尿嘧啶(5-fluorouracil,5-FU)等抗代谢药物可与TS蛋白结合,结合后的复合物不能与TS mRNA作用,导致体内TS的表达升高,是Tumour细胞产生抗药性的重要分子机制之一。
Product Picture	Sample: Bone (Mouse) Lysate at 40 ug Primary: Anti- Thymidylate synthase (SL8537R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 38 kD Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thymidylate synthase) Polyclonal Antibody, Unconjugated (SL8537R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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