TEL: +86 571 56623320 EMAIL: [email protected]
Product Name FBH1 Chinese Name F-box蛋白家族FBXO18抗体 Alias Fbx18; FLJ14590; MGC131916; MGC141935; MGC141937; F box DNA helicase 1; F box only protein 18; F box protein, helicase, 18; F-box only protein 18, F-box DNA helicase 1; FBX18_HUMAN. Research Area Cell biology immunology Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Dog, Horse, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 118kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human FBXO18 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail FBXO18 belongs to the F-box protein family, whose members share the F-box, an approximately 40 amino acid motif. It has both DNA-dependent ATPase and DNA unwinding activities.
FBXO18 is a 1,043 amino acid protein encoded by the human gene FBXO18. FBXO18 belongs to the helicase family (UvrD subfamily) and contains one F-box domain. This nuclear protein is a DNA-dependent ATPase that unwinds double-stranded DNA in a 3' to 5' direction. FBXO18 also recognizes and binds to a variety of phosphorylated proteins and promotes their ubiquitination and degradation. It also interacts with Skp1 in the SCF E3 ubiquitin ligase complex consisting of Skp1, CUL-1, Rbx1 and FBXO18.
Function:
DNA-dependent ATPase. Unwinds double-stranded DNA in a 3' to 5' direction.
Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation.
Subunit:
Part of a SCF E3 ubiquitin ligase complex consisting of SKP1, CUL1, RBX1 and FBXO18. Interacts with SKP1.
Subcellular Location:
Nuclear
Similarity:
Belongs to the helicase family. UvrD subfamily.
Contains 1 F-box domain.
SWISS:
Q8NFZ0
Gene ID:
84893
Database links:Entrez Gene: 84893 Human
GenBank: BC113377 Human
Omim: 607222 Human
SwissProt: Q8NFZ0 Human
Unigene: 498543 Human
Product Picture Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FBXO18 Polyclonal Antibody, Unconjugated(SL8492R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Scan Wechat Qrcode