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Product Name PP4R3B Chinese Name 丝氨酸/苏氨酸蛋白磷酸酶3B抗体 Alias Protein Phosphatase 4, Regulatory Subunit 3 Beta; smk1; FLFL2; PPP4R3B; PSY2; Serine/threonine-protein phosphatase 4 regulatory subunit 3B; SMEK 2; SMEK homolog 2; SMEK homolog 2 suppressor of mek1 (Dictyostelium); SMEK homolog 2 suppressor of mek1; FLJ31474; KIAA1387; PP4R3 beta; PP4R3B; PPP4R3 Beta; P4R3B_HUMAN. Research Area Tumour Cell biology immunology Developmental biology Signal transduction Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 97kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SMEK2/PP4R3B: 701-800/849 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail SMEK2 contains a WH1 domain. There are five named isoforms produced by alternative splicing. Mapping of a diet-induced hypercholesterolemia locus (Dihc2) in rats identified SMEK2 as a strong candidate for responsiveness to dietary cholesterol.
Function:
Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers.
Subunit:
Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Component of the PP4 complex PPP4C-PPP4R2-PPP4R3B.
Subcellular Location:
Cytoplasm. Cytoskeleton. Centrosome. Nucleus. Note: In interphase localized in the cytoplasm and (with higher levels) the nucleus. During metaphase located in pericentriolar regions.
Tissue Specificity:
Moderately expressed in tissues and specific brain regions examined.
Similarity:
Belongs to the SMEK family.
Contains 1 WH1 domain.
SWISS:
Q5MIZ7
Gene ID:
57223
Database links:Entrez Gene: 57223 Human
Entrez Gene: 104570 Mouse
Omim: 610352 Human
SwissProt: Q5MIZ7 Human
SwissProt: Q922R5 Mouse
Unigene: 516182 Human
Unigene: 276386 Mouse
Product Picture Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PP4R3B) Polyclonal Antibody, Unconjugated (SL8328R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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