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Product Name PA26 Chinese Name p53调控PA26核蛋白抗体 Alias p53 regulated PA26 nuclear protein; p53 regulated protein PA26; p53-regulated protein PA26; sesn1; SESN1_HUMAN; SEST1; sestrin 1; Sestrin-1. literatures Specific References (1) | SL8325R has been referenced in 1 publications.[IF=5.14] Agata Wrońska. et al. Sestrins’ Expression in the Liver Is Not Altered by Short-Term Calorie Restriction in Young and Old Rats. Gerontology. ;:1-9 WB ; Rat.Research Area Cell biology immunology Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Dog, Cow, Horse, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=3ug/Test IF=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 57kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SESN1/PA26: 401-492/492 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Cell cycle progression is subject to arrest at G1 and G2 checkpoints in response to DNA damage, presumably to allow time for DNA repair prior to entry into S and M phase, respectively. The p53 tumor suppressor is required for one such G1 checkpoint and functions to upregulate expression of GADD 45 and the mitotic inhibitory protein p21. GADD 45 stimulates DNA excision repair in vitro and inhibits entry of cells into S phase, and it apparently acts in concert with GADD 153 in inducing growth arrest. A related DNA-damage inducible gene, GADD 34 synergizes with GADD 45 or GADD 153 in suppressing cell growth. PEG-3 (progression elevated gene-3) shares significant homology with GADD 34 and is inducible by DNA damage. An additional GADD related gene, PA26, is a possible target of p53. Three isoforms of PA26 have been identified as PA26-T1, PA26-T2 and PA26-T3.
Function:
Involved in the reduction of peroxiredoxins. May also be regulator of cellular growth.
Subcellular Location:
Nucleus.
Tissue Specificity:
Widely expressed.
Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity:
Belongs to the sestrin family.
SWISS:
Q9Y6P5
Gene ID:
27244
Database links:Entrez Gene: 27244 Human
Entrez Gene: 140742 Mouse
Omim: 606103 Human
SwissProt: Q9Y6P5 Human
SwissProt: P58006 Mouse
Unigene: 591336 Human
Unigene: 139418 Mouse
Unigene: 22395 Rat
Product Picture Sample:
heart (Mouse) Lysate at 40 ug
Primary: Anti-PA26 (SL8325R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-PA26 antibody (SL8325R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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