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Product Name phospho-MYF5 (phospho Ser49) Chinese Name 磷酸化生肌决定因子Myf5抗体 Alias Myf5 (phospho S49); Myf5 (phospho Ser49); p-Myf5(phospho S49); p-Myf5(phospho Ser49); Myf-5 bHLHc2; Class C basic helix loop helix protein 2; Class C basic helix-loop-helix protein 2; Myf 5; Myf-5; Myf5; MYF5_HUMAN; Myogenic factor 5. Product Type Phosphorylated anti Research Area Cell biology immunology Developmental biology Signal transduction Stem cells Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Horse, ) Applications WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 28kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human MYF5 around the phosphorylation site of Ser49: LQG(p-S)D Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development include Myo D, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Of interest, most muscle cells express either Myo D or Myf-5 in the committed state, but when induced to differentiate, all turn on expression of myogenin. Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. Myo D-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes.
Function:
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
Subunit:
Efficient DNA binding requires dimerization with another bHLH protein.
Subcellular Location:
Nucleus.
Similarity:
Contains 1 bHLH (basic helix-loop-helix) domain.
SWISS:
P13349
Gene ID:
4617
Database links:Entrez Gene: 4617 Human
Entrez Gene: 17877 Mouse
Omim: 159990 Human
SwissProt: P13349 Human
SwissProt: P24699 Mouse
Unigene: 178023 Human
Unigene: 4984 Mouse
yf5是MyoD基因家族的一员,该家族的共同结构特点是具有1个保守的中央蛋白基序(motif),大小为80个氨基酸,称为碱性螺旋-环-螺旋(bHLH),bHLH高度保守,其中Basic结构是HLH螺旋结构的延伸,也是这一家族蛋白与DNA相互作用的区域,而HLH螺旋结构则是与其他因子相互作用的位点,即调控的重要区域。
生肌决定因子(MyoD)基因家族控制着肌细胞的增殖和分化,与肌纤维的数量、大小有着密切的关系,因而对肉质和风味有非常重要的作用,MyoD基因家族可编码4种不同的转录因子,分别为MyoD(Myf3)、Myogenin(MyoG)、Myf5、Myf6或MRF4,它们各自或协同控制着骨骼肌生成方面的关键调节因子。
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