Product Name	CCDC30
Chinese Name	卷曲螺旋结构域蛋白30抗体
Alias	CCD30_HUMAN; CCDC30; Coiled-coil domain-containing protein 30; Prefoldin subunit 6-like protein.
Research Area	Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	91kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CCDC30: 601-700/783
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Expressed in brain, kidney, pancreas, placenta, liver, thymus and prostate. Tissue Specificity: Expressed in brain, kidney, pancreas, placenta, liver, thymus and prostate. Similarity: Belongs to the prefoldin subunit beta family. SWISS: Q5VVM6 Gene ID: 728621 Database links: Entrez Gene: 728621 Human SwissProt: Q5VVM6 Human Unigene: 729640 Human
Product Picture	Sample: HepG2 Cell (Human) Lysate at 40 ug U251 Cell (Human) Lysate at 40 ug Primary: Anti-CCDC30 (SL8097R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 91 kD Observed band size: 95 kD Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min; Incubation: Anti-CCDC30 Polyclonal Antibody, Unconjugated(SL8097R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (Human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCDC30) Polyclonal Antibody, Unconjugated (SL8097R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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