Product Name	KLHL24
Chinese Name	Kelch样蛋白24抗体
Alias	DRE1; FLJ20059; FLJ25796; Kainate receptor interacting protein for GluR6; Kelch like 24 (Drosophila); Kelch like protein 24; KRIP6; OTTHUMP00000211002; OTTHUMP00000212605; Protein DRE1; KLH24_HUMAN.
Research Area	Tumour  Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Dog, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	68kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human KLHL24: 1-100/600
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	KLHL24 specifically reduces kainate receptor-mediated currents in hippocampal neurons, most probably by modulating channel properties. Function: Specifically reduces kainate receptor-mediated currents in hippocampal neurons, most probably by modulating channel properties. Subunit: Forms homodimers. Interacts with GRIK2. Subcellular Location: Perikaryon. Cell projection, axon. Cytoplasm. Similarity: Contains 1 BACK (BTB/Kelch associated) domain. Contains 1 BTB (POZ) domain. Contains 6 Kelch repeats. SWISS: Q6TFL4 Gene ID: 54800 Database links: Entrez Gene: 54800 Human Omim: 611295 Human SwissProt: Q6TFL4 Human
Product Picture	Sample: Small intestine (mouse) Lysate at 40 ug Primary: Anti- KLHL24(SL8050R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLHL24) Polyclonal Antibody, Unconjugated (SL8050R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-KLHL24 Polyclonal Antibody, Unconjugated(SL8050R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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