Product Name	CNKSR2/KSR2
Chinese Name	Ras激酶抑制因子连接增强蛋白2抗体
Alias	CNKR 2; CNK2; CNKSR2; Connector enhancer of kinase suppressor of Ras 2; Connector enhancer of KSR2; KIAA0902; KSR2; RP11 450P7.2; CNKR2_HUMAN.
Research Area	Cell biology  immunology  Signal transduction  G protein signal
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	114kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CNKSR2 corresponding to amino acids 863-508.: 481-580/1034
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	CNKR2 may function as an adapter protein or regulator of Ras signaling pathways. Function: May function as an adapter protein or regulator of Ras signaling pathways. Subunit: Interacts with RAF1, RAB2L and RAL GTPase proteins. Subcellular Location: Cytoplasm. Membrane; Peripheral membrane protein. Post-translational modifications: Phosphorylated on tyrosine. Similarity: Belongs to the CNKSR family. Contains 1 CRIC domain. Contains 1 DUF1170 domain. Contains 1 PDZ (DHR) domain. Contains 1 PH domain. Contains 1 SAM (sterile alpha motif) domain. SWISS: Q8WXI2 Gene ID: 22866 Database links: Entrez Gene: 22866 Human Entrez Gene: 245684 Mouse Entrez Gene: 59322 Rat SwissProt: Q8WXI2 Human SwissProt: Q80YA9 Mouse SwissProt: Q9Z1T4 Rat Unigene: 632794 Human Unigene: 197074 Mouse
Product Picture	Sample: U251(Human) Cell Lysate at 30 ug Primary: Anti-CNKSR2/KSR2 (SL8029R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 114 kD Observed band size: 114 kD Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CNKSR2/KSR2) Polyclonal Antibody, Unconjugated (SL8029R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CNKSR2/KSR2) Polyclonal Antibody, Unconjugated (SL8029R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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