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Product Name ANKRD9 Chinese Name 锚蛋白重复结构域蛋白9抗体 Alias Ankyrin repeat domain-containing protein 9; 2500003O20Rik; ANKR9_HUMAN; Ankrd9; Ankyrin repeat domain 9; MGC21990. Research Area Cell biology immunology Immunogen Species Rabbit Clonality Polyclonal React Species Human, Rat, (predicted: Mouse, Dog, Pig, Cow, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 34kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ANKRD9: 21-200/317 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail ANKRD9 is a 317 amino acid protein that contains three ANK repeats. Encoded by a gene that maps to human chromosome 14q32.31, ANKRD9 is conserved in chimpanzee, dog, cow, mouse, rat and zebrafish. Hepatic mRNA levels of ANKRD9 are repressed by both thyroid hormone (T(3)) and fasting, and re-elevate by feeding after fasting. ANKRD9 mRNA levels also decrease in response to apoptosis. Localizing to cytoplasm, ANKRD9 may be involved in intracellular lipid accumulation and lipid metabolism. ANKRD9 may also function as a molecular chaperone
Similarity:
Contains 3 ANK repeats.
SWISS:
Q96BM1
Gene ID:
122416
Database links:
Entrez Gene: 122416 Human
Entrez Gene: 74251 Mouse
SwissProt: Q96BM1 Human
SwissProt: Q8BH83 Mouse
Unigene: 432945 Human
Unigene: 250989 Mouse
Product Picture Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti- ANKRD9 (SL7964R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 35 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ANKRD9 Polyclonal Antibody, Unconjugated(SL7964R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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