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Rabbit Anti-GAK protein antibody
Rabbit Anti-GAK protein antibody
Cyclin G associated kinase; Cyclin-G-associated kinase; EC 2.7.11.1;FLJ16629; FLJ40395; GAK; GAK protein; GAK_HUMAN; MGC99654.
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  • NO.:SL7912R
    Clonality:Polyclonal
    Immunogen Species:Rabbit
    React Species:Mouse,(predicted: Human,Rat,Chicken,Dog,Cow,Horse,)
    Applications:WB ELISA IHC-P IHC-F IF
    concentration:1mg/ml
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Details

Product Name GAK protein
Chinese Name CyclinG相关激酶抗体
Alias Cyclin G associated kinase; Cyclin-G-associated kinase; EC 2.7.11.1; FLJ16629; FLJ40395; GAK; GAK protein; GAK_HUMAN; MGC99654.  
Research Area Cell biology  Neurobiology  Signal transduction  Cyclin  Kinases and Phosphatases  
Immunogen Species Rabbit
Clonality Polyclonal
React Species Mouse,  (predicted: Human, Rat, Chicken, Dog, Cow, Horse, )
Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 144kDa
Cellular localization cytoplasmic The cell membrane 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human GAK protein: 244-330/1311 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.
Tissue specificity: Ubiquitous. Highest in testis.

Function:
Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.

Subcellular Location:
Cytoplasm, perinuclear region. Golgi apparatus, trans-Golgi network. Cell junction, focal adhesion (Probable). Note=Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focals adhesions.

Tissue Specificity:
Ubiquitous. Highest in testis.

Similarity:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 C2 tensin-type domain.
Contains 1 J domain.
Contains 1 phosphatase tensin-type domain.
Contains 1 protein kinase domain.

SWISS:
O14976

Gene ID:
2580

Product Picture
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Large intestine (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Primary: Anti- GAK protein (SL7912R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 25 kD

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