TEL: +86 571 56623320 EMAIL: [email protected]
Product Name PPHLN1 Chinese Name 脉周蛋白1抗体(胃癌抗原蛋白Ga50) Alias Gastric cancer antigen Ga50; Periphilin 1; Periphilin-1; PPHLN_HUMAN; PPHLN1. Research Area Cell biology immunology Cyclin Cell differentiation Cell type markers Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 53kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human PPHLN1: 301-400/458 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Involved in epithelial differentiation and contributes to epidermal integrity and barrier formation.
Function:
Involved in epithelial differentiation and contributes to epidermal integrity and barrier formation.
Subunit:
Homodimer. Interacts with PPL.
Subcellular Location:
Nucleus. Cytoplasm.
Tissue Specificity:
Ubiquitous.
Post-translational modifications:
Substrate of transglutaminase (in vitro).
SWISS:
Q8NEY8
Gene ID:
51535
Database links:
UniProtKB/Swiss-Prot: Q8NEY8.2
The protein encoded by this gene is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008].Product Picture Sample:
U937(Human) Cell Lysate at 30 ug
Primary: Anti- PPHLN1 (SL7872R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Sample:
HL60 (Human) Cell Lysate at 30 ug
Primary: Anti- PPHLN1 (SL7872R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Paraformaldehyde-fixed, paraffin embedded (rat skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPHLN1) Polyclonal Antibody, Unconjugated (SL7872R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPHLN1) Polyclonal Antibody, Unconjugated (SL7872R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Scan Wechat Qrcode